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Split TEV information


The split TEV technique[1] is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of two previously inactive fragments derived from the NIa protease of the tobacco etch virus (TEV protease). These fragments, either an N-terminal (NTEV) or C-terminal part (CTEV), are fused to protein interaction partners of choice. Upon interaction of the two candidate proteins, the NTEV and CTEV fragments get into close proximity, regain proteolytic activity, and activate specific TEV reporters which indicate an occurred protein-protein interaction.

  1. ^ Wehr, Michael C; Laage, Rico; Bolz, Ulrike; Fischer, Tobias M; Grünewald, Sylvia; Scheek, Sigrid; Bach, Alfred; Nave, Klaus-Armin; Rossner, Moritz J (2006). "Monitoring regulated protein-protein interactions using split TEV". Nature Methods. 3 (12): 985–93. doi:10.1038/nmeth967. PMID 17072307.

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Split TEV

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The split TEV technique is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of...

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TEV protease

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TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus...

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Movement for a Democratic Society

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The Movement for a Democratic Society (Kurdish: Tevgera Civaka Demokratîk, TEV-DEM, Arabic: حركة المجتمع الديمقراطي, Classical Syriac: ܙܘܥܐ ܕܟܢܫܐ ܕܝܡܩܪܐܛܝܐ...

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Split supersymmetry

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matter candidate, that split supersymmetry is one amongst a few theories that exists. The new light (~TeV) particles in Split Supersymmetry (beyond the...

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Large extra dimensions

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[clarification needed] The size of the dimensions in ADD is around the order of the TeV scale, which results in it being experimentally probeable by current colliders...

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List of accelerators in particle physics

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Culture in Erice, Sicily. The center-of-mass energy was planned to be 200 TeV, and the size was planned to span parts of Europe and Asia. Fermitron was...

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Higgs boson

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initially at energies of 3.5 TeV per beam (7 TeV total), or almost 3.6 times that of the Tevatron, and upgradeable to 2 × 7 TeV (14 TeV total) in future. Theory...

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Gravitino

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cosmological gravitino problem is the split supersymmetry model, where the gravitino mass is much higher than the TeV scale, but other fermionic supersymmetric...

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Electromagnetic spectrum

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M.; Samuelson, F. W.; Saz Parkinson, P. M.; et al. (2007). "Discovery of TeV Gamma-Ray Emission from the Cygnus Region of the Galaxy". The Astrophysical...

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Total economic value

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Total economic value (TEV) is a concept in cost–benefit analysis that refers to the value derived by people from a natural resource, a man-made heritage...

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Savas Dimopoulos

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and extra spatial dimensions, which have shaped theoretical research on TeV-scale physics, thereby inspiring a wide range of experiments." In 2006, he...

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Superstring theory

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of the Minimal Supersymmetric Standard Model squarks has been up to 1.1 TeV, and gluinos up to 500 GeV. No report on suggesting large extra dimensions...

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PA clan of proteases

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In addition to the double β-barrel core, some viral proteases (such as TEV protease) have a long, flexible C-terminal loop that forms a lid that completely...

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ATLAS experiment

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the LHC energy has been increasing: 1.8 TeV at the end of 2009, 7 TeV for the whole of 2010 and 2011, then 8 TeV in 2012. The first data-taking period performed...

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Autonomous Administration of North and East Syria

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Unopposed, the PYD's political coalition, Movement for a Democratic Society (TEV-DEM), controlled the Kurdish Supreme Committee until the latter was dissolved...

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Kurdish Future Movement in Syria

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assassinated. Following internal leadership battles, the party has suffered a split, with both factions continuing to exist under the same name. The Kurdish...

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Kurdistan Democratic Party of Syria

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rename both entities. Nasr al-Din Ibrahim was responsible for the fourth split. The eighth party conference was marked by intense disagreements between...

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Supersymmetry

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that it must break supersymmetry permanently and must give superparticles TeV scale masses. There are many models that can do this and most of their details...

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Missing energy

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the CMS missing transverse momentum reconstruction in pp data at √ s = 8 TeV". Journal of Instrumentation. 10 (2): P02006. arXiv:1411.0511. Bibcode:2015JInst...

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Protein tag

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purification, tags are sometimes removed by specific proteolysis (e.g. by TEV protease, Thrombin, Factor Xa or Enteropeptidase) or intein splicing. (See...

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Depleted uranium

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(1983). An Experiment at D0 to Study anti-Proton – Proton Collisions at 2-TeV: Design Report (Report). Office of Scientific and Technical Information (OSTI)...

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Religion

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Religions of the World: a Latter-day Saint [Mormon] View. 2nd general ed., tev. and enl. Provo, Utah: Brigham Young University, 1997. xv, 294 p., ill. ISBN 0-8425-2350-2...

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Trondheim Energi

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the company was split into Trondheim Energiverk (TEV) and Trondheim Sporvei, the latter with responsibility for the tramway. In 1952 TEV and Stockholm Elverk...

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Latvian language

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According to some glottochronological speculations, the East Baltic languages split from West Baltic (or, perhaps, from the hypothetical proto-Baltic language)...

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Minimal Supersymmetric Standard Model

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be 125–126 GeV. If the superpartners of the Standard Model are near the TeV scale, then measured gauge couplings of the three gauge groups unify at high...

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