Pilus-related Sortase C of Group B Streptococcus. PDB entry 3O0P[1]
Identifiers
Symbol
Sortase
Pfam
PF04203
InterPro
IPR005754
SCOP2
1ija / SCOPe / SUPFAM
OPM superfamily
294
OPM protein
1rz2
CDD
cd00004
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
Sortase refers to a group of prokaryotic enzymes that modify surface proteins by recognizing and cleaving a carboxyl-terminal sorting signal. For most substrates of sortase enzymes, the recognition signal consists of the motif LPXTG (Leu-Pro-any-Thr-Gly), then a highly hydrophobic transmembrane sequence, followed by a cluster of basic residues such as arginine. Cleavage occurs between the Thr and Gly, with transient attachment through the Thr residue to the active site Cys residue, followed by transpeptidation that attaches the protein covalently to cell wall components. Sortases occur in almost all Gram-positive bacteria and the occasional Gram-negative bacterium (e.g. Shewanella putrefaciens) or Archaea (e.g. Methanobacterium thermoautotrophicum), where cell wall LPXTG-mediated decoration has not been reported.[2][3] Although sortase A, the "housekeeping" sortase, typically acts on many protein targets, other forms of sortase recognize variant forms of the cleavage motif, or catalyze the assembly of pilins into pili.[4][5][6]
^Cozzi R, Malito E, Nuccitelli A, D'Onofrio M, Martinelli M, Ferlenghi I, Grandi G, Telford JL, Maione D, Rinaudo CD (June 2011). "Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus". FASEB Journal. 25 (6): 1874–86. doi:10.1096/fj.10-174797. hdl:11562/349253. PMID 21357525. S2CID 28182632.
^Mazmanian SK, Ton-That H, Schneewind O (June 2001). "Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus". Molecular Microbiology. 40 (5): 1049–57. CiteSeerX 10.1.1.513.4509. doi:10.1046/j.1365-2958.2001.02411.x. PMID 11401711. S2CID 34467346.
^Pallen MJ, Chaudhuri RR, Henderson IR (October 2003). "Genomic analysis of secretion systems". Current Opinion in Microbiology. 6 (5): 519–27. doi:10.1016/j.mib.2003.09.005. PMID 14572546.
^Oh SY, Budzik JM, Schneewind O (September 2008). "Sortases make pili from three ingredients". Proceedings of the National Academy of Sciences of the United States of America. 105 (37): 13703–4. Bibcode:2008PNAS..10513703O. doi:10.1073/pnas.0807334105. PMC 2544515. PMID 18784365.
^LeMieux J, Woody S, Camilli A (September 2008). "Roles of the sortases of Streptococcus pneumoniae in assembly of the RlrA pilus". Journal of Bacteriology. 190 (17): 6002–13. doi:10.1128/JB.00379-08. PMC 2519520. PMID 18606733.
^Kang HJ, Coulibaly F, Proft T, Baker EN (January 2011). Hofmann A (ed.). "Crystal structure of Spy0129, a Streptococcus pyogenes class B sortase involved in pilus assembly". PLOS ONE. 6 (1): e15969. Bibcode:2011PLoSO...615969K. doi:10.1371/journal.pone.0015969. PMC 3019223. PMID 21264317.
Sortase refers to a group of prokaryotic enzymes that modify surface proteins by recognizing and cleaving a carboxyl-terminal sorting signal. For most...
Sortase A (EC 3.4.22.70, SrtA, SrtA protein, SrtA sortase) is an enzyme. This enzyme catalyses a cell wall sorting reaction, in which a surface protein...
Sortases are membrane anchored enzyme that sort these surface proteins onto the bacterial cell surface and anchor them to the peptidoglycan. There are...
Nikghalb, Kevyan D. (2018). "Expanding the Scope of Sortase-Mediated Ligations by Using Sortase Homologues". ChemBioChem. 19 (7): 185–195. doi:10.1002/cbic...
aspect that distinguishes sortases in general is that they have a very specific targeting for their substrate, as sortases have generally two functions...
include the use of split inteins, spontaneous isopeptide bond formation and sortase ligation. In order to optimize synthesis of long peptides, a method was...
(hence the name "toxin mediated pilus"). Bacterial nanowires Flagellum Sortase P fimbriae PilZ domain "pilus" at Dorland's Medical Dictionary Brinton...
enzyme D-glutamyl transpeptidase A protein-sorting transpeptidase (e.g. sortase), that cleaves a C-terminal sorting signal from its target protein(s) and...
pentaglycine bridges (chains of five glycine residues) by the transpeptidase sortase A. Protein A, an IgG-binding protein, binds to the Fc region of an antibody...
C15 Pyroglutamyl-peptidase I (Bacillus amyloliquefaciens) CL C60, C82 Sortase A (Staphylococcus aureus) CM C18 Hepatitis C virus peptidase 2 (hepatitis...
enzyme responsible of linking such proteins to cell wall, that is the Sortase A (SrtA), rather than any single surface protein involved in the mechanism...
C15 Pyroglutamyl-peptidase I (Bacillus amyloliquefaciens) CL C60, C82 Sortase A (Staphylococcus aureus) CM C18 Hepatitis C virus peptidase 2 (hepatitis...
covalent attachment to the bacterial cell wall. A specialized enzyme, sortase, cleaves the target protein at a characteristic recognition site near the...
signal PEP-CTERM. The name derives from a predicted role analogous to sortase, despite the lack of any detectable sequence homology, and a strong association...
bacteria are cleaved between the T and G residue in the LPXTG sortase signal by the enzyme sortase (the enzyme was not discovered yet, but was later shown by...
anchor motif of surface proteins similar to Sortase. However, LPXTGase differs significantly from Sortase in several ways: a) it is glycosylated, b) it...
step. An early example, involved the stabilization of the transpeptidase Sortase A which resulted in INCYPRO-stabilized variants with activity under elevated...
antigens from the Wuhan strain. Another candidate is RBD–scNP, which is a sortase A-conjugated ferritin nanoparticle with receptor-binding domain (RBD) antigens...
virulence factors include factors required for biofilm formation (e.g. sortases) and integrins (e.g. beta-1 and 3). Strategies to target virulence factors...
University of Chicago investigating the biochemistry and biological role of sortase, a transpeptidase that links surface proteins to the envelope of Gram-positive...
and Tollway Authority, a government agency of the U.S. state of Georgia Sortase A, an enzyme, abbreviated as SrtA This disambiguation page lists articles...
data. This helped lead to the identification of the anchor transpeptidase sortase by Olaf Schneewind, a former member of his lab. By the late 1990s, Fischetti...
Endospores. [H] Gram-positive Bacteria: hypertrophy of the wall peptidoglycan, sortase enzyme and a loss of the outer membrane. [I] Glycerol 1-P dehydrogenase...
Smoothened (SMO). TDsmURFP was purified from E. coli and attached to SMO by sortase-mediated conjugation for fluorescence-activated cell sorting (FACS). This...
gram-positive cocci. They are named for a C-terminal motif used by the sortase. There is also a LPXTGase. LPXTG Pili in Gram-positive bacteria contain...
protein-sorting domain. Thus archaeosortase appears to be a transpeptidase, like sortase, rather than a simple protease. Archaeosortases are related to exosortases...
protein labeling technique, the researchers used the bacterial enzyme sortase A to establish a strong chemical bond between the surface protein and a...
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