Crystal structure of the E.coli SeqA protein complexed with n6-methyladenine- guanine mismatch DNA
Identifiers
Symbol
SeqA
Pfam
PF03925
InterPro
IPR005621
SCOP2
1lrr / SCOPe / SUPFAM
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
In molecular biology the SeqA protein is found in bacteria and archaea. The function of this protein is highly important in DNA replication. The protein negatively regulates the initiation of DNA replication at the origin of replication, in Escherichia coli, OriC.[1] Additionally the protein plays a further role in sequestration. The importance of this protein is vital, without its help in DNA replication, cell division and other crucial processes could not occur. This protein domain is thought to be part of a much larger protein complex which includes other proteins such as SeqB.[2]
^Slater S, Wold S, Lu M, Boye E, Skarstad K, Kleckner N (September 1995). "E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration". Cell. 82 (6): 927–36. doi:10.1016/0092-8674(95)90272-4. PMID 7553853. S2CID 14652024.
^Shakibai N, Ishidate K, Reshetnyak E, Gunji S, Kohiyama M, Rothfield L (September 1998). "High-affinity binding of hemimethylated oriC by Escherichia coli membranes is mediated by a multiprotein system that includes SeqA and a newly identified factor, SeqB". Proceedings of the National Academy of Sciences of the United States of America. 95 (19): 11117–21. Bibcode:1998PNAS...9511117S. doi:10.1073/pnas.95.19.11117. PMC 21605. PMID 9736699.
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