Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene.[5][6][7] STIM1 has a single transmembrane domain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane.[8]
Even though the protein has been identified earlier, its function was unknown until recently. In 2005, it was discovered that STIM1 functions as a calcium sensor in the endoplasmic reticulum.[9][10] Upon activation of the IP3 receptor, the calcium concentration in the endoplasmic reticulum decreases, which is sensed by STIM1, via its EF hand domain. STIM1 activates the "store-operated" ORAI1 calcium ion channels in the plasma membrane, via intracellular STIM1 movement, clustering under plasma membrane and protein interaction with ORAI isoforms.[11][12][13] STIM1-mediated calcium entry is required for thrombin-induced disassembly of VE-cadherin adherens junctions.[14] 2-Aminoethoxydiphenyl borate (2-APB) and 4-chloro-3-ethylphenol (4-CEP) cause STIM1 clustering in a cell and prevent STIM1 moving toward plasma membrane.[15]
^ abcGRCh38: Ensembl release 89: ENSG00000167323 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000030987 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Parker NJ, Begley CG, Smith PJ, Fox RM (Oct 1996). "Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5". Genomics. 37 (2): 253–6. doi:10.1006/geno.1996.0553. PMID 8921403.
^Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA (Aug 2001). "Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins". The Biochemical Journal. 357 (Pt 3): 673–85. doi:10.1042/0264-6021:3570673. PMC 1221997. PMID 11463338.
^Williams RT, Senior PV, Van Stekelenburg L, Layton JE, Smith PJ, Dziadek MA (Apr 2002). "Stromal interaction molecule 1 (STIM1), a transmembrane protein with growth suppressor activity, contains an extracellular SAM domain modified by N-linked glycosylation". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1596 (1): 131–7. doi:10.1016/S0167-4838(02)00211-X. PMID 11983428.
^Roos J, DiGregorio PJ, Yeromin AV, Ohlsen K, Lioudyno M, Zhang S, Safrina O, Kozak JA, Wagner SL, Cahalan MD, Veliçelebi G, Stauderman KA (May 2005). "STIM1, an essential and conserved component of store-operated Ca2+ channel function". The Journal of Cell Biology. 169 (3): 435–45. doi:10.1083/jcb.200502019. PMC 2171946. PMID 15866891.
^Liou J, Kim ML, Heo WD, Jones JT, Myers JW, Ferrell JE, Meyer T (Jul 2005). "STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx". Current Biology. 15 (13): 1235–41. doi:10.1016/j.cub.2005.05.055. PMC 3186072. PMID 16005298.
^Zhou Y, Srinivasan P, Razavi S, Seymour S, Meraner P, Gudlur A, Stathopulos PB, Ikura M, Rao A, Hogan PG (Aug 2013). "Initial activation of STIM1, the regulator of store-operated calcium entry". Nature Structural & Molecular Biology. 20 (8): 973–81. doi:10.1038/nsmb.2625. PMC 3784406. PMID 23851458.
^Ma G, Wei M, He L, Liu C, Wu B, Zhang SL, Jing J, Liang X, Senes A, Tan P, Li S, Sun A, Bi Y, Zhong L, Si H, Shen Y, Li M, Lee MS, Zhou W, Wang J, Wang Y, Zhou Y (2015-07-17). "Inside-out Ca(2+) signalling prompted by STIM1 conformational switch". Nature Communications. 6: 7826. Bibcode:2015NatCo...6.7826M. doi:10.1038/ncomms8826. PMC 4509486. PMID 26184105.
^Soni D, Regmi SC, Wang DM, DebRoy A, Zhao YY, Vogel SM, Malik AB, Tiruppathi C (Apr 2017). "Pyk2 Phosphorylation of VE-PTP Downstream of STIM1 induced Ca2+ entry Regulates Disassembly of Adherens Junctions". American Journal of Physiology. Lung Cellular and Molecular Physiology. 312 (6): L1003–L1017. doi:10.1152/ajplung.00008.2017. PMC 5495943. PMID 28385807.
^Zeng B, Chen GL, Xu SZ (Oct 2012). "Store-independent pathways for cytosolic STIM1 clustering in the regulation of store-operated Ca(2+) influx". Biochemical Pharmacology. 84 (8): 1024–35. doi:10.1016/j.bcp.2012.07.013. PMID 22842488.
interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembrane domain, and is localized to the endoplasmic...
channel. ORAI1 interacts with the STIM1 protein. STIM1 is a transmembrane protein of the endoplasmic reticulum (ER). STIM1 can sense the concentration of...
calcium concentration in the endoplasmic reticulum is sensed by the STIM1 protein. STIM1 clusters upon the depletion of the calcium stores and forms "puncta"...
(STIM) family which comprises only two members together with its homologue STIM1, and likely arose from a common ancestral gene. They encode type 1 transmembrane...
peptide and the transmembrane domain derived from STIM1, with GFP placed in between as a reporter. STIM1 is an ER-resident calcium sensor protein responsible...
vertebrates. STIMATE can physically associate with STIM1 to promote conformational switch of STIM1 from inactive toward an activated state, thereby coupling...
calcium into the cytosol. Low calcium in the endoplasmic reticulum causes STIM1 clustering on the ER membrane and leads to activation of cell membrane CRAC...
and Orais, however, have more recently been implicated in this process. STIM1 and TRPC1 can coassemble, complicating the understanding of this phenomenon...
ICRAC occurs are currently still under investigation. Although Orai1 and STIM1, have been linked by several studies, for a proposed model of store-operated...
configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene. Click on genes...
containing ER Ca2+ sensor protein, stromal interaction molecule 1 and 2 (STIM1, STIM2), has been shown to contain a Ca2+-binding canonical EF-hand motif...
ORAI1 is a molecular component of the CRAC, and it interacts with STIM1 an ER protein. STIM1 can rapidly translocate to a PM-ER contact site after depletion...
shown that tyrosine phosphorylation of VE-PTP via Pyk2 kinase downstream of STIM1-induced calcium entry mediates disassembly of the endothelial adherens junctions...
endoplasmic reticulum (ER) membrane resident protein that associates with STIM1, to facilitate the inactivation of SOCE. SARAF plays a key role in shaping...
Tiruppathi C (Apr 2017). "Pyk2 Phosphorylation of VE-PTP Downstream of STIM1 induced Ca2+ entry Regulates Disassembly of Adherens Junctions". American...
spine, leading to SA calcium depletion. Calcium is finally refilled by ORAI-STIM1 mostly located in clusters in the spine head. The slow calcium influx through...
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