Stimulated emission depletion (STED) microscopy provides significant resolution improvements over those possible with Confocal microscopy.
Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of fluorophores, minimizing the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system.[1] It was developed by Stefan W. Hell and Jan Wichmann in 1994,[2] and was first experimentally demonstrated by Hell and Thomas Klar in 1999.[3] Hell was awarded the Nobel Prize in Chemistry in 2014 for its development. In 1986, V.A. Okhonin[4] (Institute of Biophysics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk) had patented the STED idea.[5] This patent was unknown to Hell and Wichmann in 1994.
STED microscopy is one of several types of super resolution microscopy techniques that have recently been developed to bypass the diffraction limit of light microscopy to increase resolution. STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to achieve an improvement in resolution, that is to say STED allows for images to be taken at resolutions below the diffraction limit. This differs from the stochastic functional techniques such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) as these methods use mathematical models to reconstruct a sub diffraction limit from many sets of diffraction limited images.
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Westphal, V.; S. O. Rizzoli; M. A. Lauterbach; D. Kamin; R. Jahn; S. W. Hell (2008). "Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement". Science. 320 (5873): 246–249. Bibcode:2008Sci...320..246W. doi:10.1126/science.1154228. PMID 18292304. S2CID 14169050.
^Cite error: The named reference STED13 was invoked but never defined (see the help page).
^Klar, Thomas A.; Stefan W. Hell (1999). "Subdiffraction resolution in far-field fluorescence microscopy". Optics Letters. 24 (14): 954–956. Bibcode:1999OptL...24..954K. doi:10.1364/OL.24.000954. PMID 18073907.
^"Victor Okhonin".
^Okhonin V.A., A method of examination of sample microstructure, Patent SU 1374922, (See also in the USSR patents database SU 1374922) priority date April 10, 1986, Published on July 30, 1991, Soviet Patents Abstracts, Section EI, Week 9218, Derwent Publications Ltd., London, GB; Class S03, p. 4. Cited by patents US 5394268 A (1993) and US RE38307 E1 (1995). From the English translation: "The essence of the invention is as follows. Luminescence is excited in a sample placed in the field of several standing light waves, which cause luminescence quenching because of stimulated transitions...".
Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by...
4Pi-STED-microscope is the result of combining the two unrelated concepts of Stimulated emission depletion (STED) microscopy and 4Pi-microscopy. Here...
microscope (SNOM/NSOM), STEDmicroscopy (STED), and scanning electron microscopy and electrochemical AFM, EC-AFM). Although SNOM and STED use visible, infrared...
STED may refer to: STEDmicroscopy (stimulated emission depletion) STED sewer (septic tank effluent drainage) Septic tank effluent rainage system, a type...
super-resolution microscopy. These include optical near-fields (Near-field scanning optical microscope) or a diffraction technique called 4Pi STEDmicroscopy. Objects...
imaged with conventional or confocal microscopy. Within RESOLFT the principles of STEDmicroscopy and GSD microscopy are generalized. Structures that are...
diffraction limit is possible, but it has major limitations. STED is a fluorescence microscopy technique which uses a combination of light pulses to induce...
first technique to really achieve a sub-diffraction resolution was STEDmicroscopy, proposed in 1994. This method and all techniques following the RESOLFT...
where he developed the principle for stimulated emission depletion STEDmicroscopy. From 1993 to 1994 Hell was also for six months a visiting scientist...
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique...
diffraction limits using a technique called Stimulated Emission Depletion (STED) Microscopy. This technique takes advantage of the low intensity at the singularity...
two to four times and techniques like stimulated emission depletion (STED) microscopy are approaching the resolution of electron microscopes. This occurs...
combination of stimulated emission depletion microscopy (STED) and selective plane illumination microscopy has been published, that leads to a reduced...
studied hearing and vision Stefan Hell — developed the principle of STEDmicroscopy Richard Henderson — scientist at the MRC Laboratory of Molecular Biology...
Conventional light microscopy is constrained by light diffraction. Researchers have recently used stimulated emission depletion (STED) microscopy, a super-resolution...
1988: Discovery of giant magnetoresistance by Peter Grünberg 1994: STEDmicroscopy by Stefan Hell and Jan Wichmann 1998: Frequency comb by Theodor W....
nanocrystals have been utilized to achieve high-resolution imaging in STEDmicroscopy. This technique involves exciting the fluorescent probe with an excitation...
optical microscopy they produce commercial versions of the STED microscope offering sub-diffraction resolution. In 2007 they launched the TCS STED which...
has made major contributions to advanced microscopy, particularly superresolution microscopy (RESOLFT, STED).[citation needed] Testa studied physics at...
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