The rpoB gene encodes the β subunit of bacterial RNA polymerase and the homologous plastid-encoded RNA polymerase (PEP). It codes for 1342 amino acids in E. coli, making it the second-largest polypeptide in the bacterial cell.[1] It is targeted by the rifamycin family of antibacterials, such as rifampin.[2] Mutations in rpoB that confer resistance to rifamycins do so by altering the protein's drug-binding residues, thereby reducing affinity for these antibiotics.[3][4]
Some bacteria contain multiple copies of the 16S rRNA gene, which is commonly used as the molecular marker to study phylogeny. In these cases, the single-copy rpoB gene can be used to study microbial diversity.[5][6]
An inhibitor of transcription in bacteria, tagetitoxin, also inhibits PEP, showing that the complex found in plants is very similar to the homologous enzyme in bacteria. [7]
^Goldstein BP (September 2014). "Resistance to rifampicin: a review". The Journal of Antibiotics. 67 (9): 625–30. doi:10.1038/ja.2014.107. PMID 25118103.
^Floss HG, Yu TW (February 2005). "Rifamycin-mode of action, resistance, and biosynthesis". Chemical Reviews. 105 (2): 621–32. doi:10.1021/cr030112j. PMID 15700959.
^Campbell EA, Korzheva N, Mustaev A, Murakami K, Nair S, Goldfarb A, Darst SA (March 2001). "Structural mechanism for rifampicin inhibition of bacterial rna polymerase". Cell. 104 (6): 901–12. doi:10.1016/S0092-8674(01)00286-0. PMID 11290327. S2CID 8229399.
^Feklistov A, Mekler V, Jiang Q, Westblade LF, Irschik H, Jansen R, Mustaev A, Darst SA, Ebright RH (September 2008). "Rifamycins do not function by allosteric modulation of binding of Mg2+ to the RNA polymerase active center". Proceedings of the National Academy of Sciences of the United States of America. 105 (39): 14820–5. Bibcode:2008PNAS..10514820F. doi:10.1073/pnas.0802822105. PMC 2567451. PMID 18787125.
^Case RJ, Boucher Y, Dahllöf I, Holmström C, Doolittle WF, Kjelleberg S (January 2007). "Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies". Applied and Environmental Microbiology. 73 (1): 278–88. Bibcode:2007ApEnM..73..278C. doi:10.1128/AEM.01177-06. PMC 1797146. PMID 17071787.
^Vos M, Quince C, Pijl AS, de Hollander M, Kowalchuk GA (2012). "A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity". PLOS ONE. 7 (2): e30600. Bibcode:2012PLoSO...730600V. doi:10.1371/journal.pone.0030600. PMC 3280256. PMID 22355318.
^Börner T, Aleynikova AY, Zubo YO, Kusnetsov VV (September 2015). "Chloroplast RNA polymerases: Role in chloroplast biogenesis". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1847 (9): 761–9. doi:10.1016/j.bbabio.2015.02.004. PMID 25680513.
The rpoB gene encodes the β subunit of bacterial RNA polymerase and the homologous plastid-encoded RNA polymerase (PEP). It codes for 1342 amino acids...
map to the rpoB gene, encoding the beta subunit of RNA polymerase. The majority of resistance mutations in E. coli are in 3 clusters on rpoB. Cluster I...
g., rpo genes encode RNA polymerase). To distinguish among different alleles, the abbreviation is followed by an upper-case letter (e.g., the rpoB gene...
The Royal Philharmonic Orchestra (RPO) is a British symphony orchestra based in London. The RPO was established by Thomas Beecham in 1946. In its early...
mutations in the rifampin-resistance determining region (RRDR) within the rpoB gene. The most frequently observed mutations of the codons in RRDR are 531...
The gene rpoS (RNA polymerase, sigma S, also called katF) encodes the sigma factor sigma-38 (σ38, or RpoS), a 37.8 kD protein in Escherichia coli. Sigma...
Wollinella. Additionally, a rare genetic event has led to the fusion of the rpoB and rpoC genes in this family, which is characteristic of them. Recently, new...
2108–12. PMC 265452. PMID 1500518. Korczak, B. (1 July 2004). "Phylogeny of the family Pasteurellaceae based on rpoB sequences". International Journal of Systematic...
approach, based on partial sequencing of protein-encoding genes (gyrB, rpoB, infB and atpD), this species has been reclassified to the genus Kosakonia...
Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences". Journal of Clinical Microbiology. 46 (3):...
monophyletic clade in a phylogenetic tree constructed using RpoB, RpoC, and DNA gyrase B protein sequences. This clade is also strongly supported by a...
supported by CSIs in a number of important proteins (viz. Hsp70, Hsp60, RpoB, RpoB and AlaRS), which support its placement in the proximity of the phylum...
the diversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR-DGGE method". FEMS Microbiology Ecology. 53 (2): 317–28. doi:10...
16S rRNA, though both species can be differentiated by their hsp65, ITS or rpoB gene sequences.[citation needed] A draft genome sequence of M. abscessus...
Holmström C, Doolittle WF, Kjelleberg S (January 2007). "Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies". Applied and Environmental...
Regular Production Option (RPO) is a 3-digit standardized code used by General Motors to designate vehicle options & modifications. RPO codes designate how a...
Gwendolyn L. (2014-05-01). "Rolling Circle Amplification for Direct Detection of rpoB Gene Mutations in Mycobacterium tuberculosis Isolates from Clinical Specimens"...
chloroplasts. β The β subunit is the second-largest subunit, and is encoded by the rpoB gene. The β subunit contains the rest of the active center responsible for...
already identified. Among them, 16S rRNA, 23S rRNA, RNA polymerase subunit B (rpoB), and manganese-dependent superoxide dismutase (sodA) are used to study...
as Klebsiella pneumoniae. Phylogenic comparisons between the 16s rRNA and rpoB genes of this and other Klebsiella species have suggested classification...
proteins such as RpoB, RpoC, UvrD, GyrA, PolA, PolC, MraW, NadD, PyrE, RpsA, RpsH, FtsA, RadA, etc., including a large >300 aa insert in the RpoC protein, are...
Microbiology: Corynebacterium [1] Khamis, A.; Raoult, D.; Scola, B. La (2004). "rpoB gene sequencing for identification of Corynebacterium species". Journal...
are presence of styles with glabrous style branches and a deletion in the rpoB gene. Two tribes, Wunderlicheae and Hyalideae, are recognised. Panero, J...
An example is the N1177 strain, which carries a single mutation in the rpoB gene that confers resistance to rifampicin that modified transcription levels...
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