Polysome profiling is a technique in molecular biology that is used to study the association of mRNAs with ribosomes. It is important to note that this technique is different from ribosome profiling. Both techniques have been reviewed[1] and both are used in analysis of the translatome, but the data they generate are at very different levels of specificity. When employed by experts, the technique is remarkably reproducible: the 3 profiles in the first image are from 3 different experiments.[2]
^Piccirillo, CA; et al. (2014). "Translational control of immune responses: from transcripts to translatomes". Nature Immunology. 15 (6): 503–511. doi:10.1038/ni.2891. PMID 24840981. S2CID 6269940.
^Hanebuth, MA; et al. (2016). "Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction". Nature Communications. 7: 13695. Bibcode:2016NatCo...713695H. doi:10.1038/ncomms13695. PMC 5150220. PMID 27917864.
and 23 Related for: Polysome profiling information
Polysomeprofiling is a technique in molecular biology that is used to study the association of mRNAs with ribosomes. It is important to note that this...
A polyribosome (or polysome or ergosome) is a group of ribosomes bound to an mRNA molecule like “beads” on a “thread”. It consists of a complex of an mRNA...
differences in protein synthesis. Other methods are polysomeprofiling, full-length translating mRNA profiling (RNC-seq), and translating ribosome affinity purification...
present in a sample. This technique is also different from polysomeprofiling. Ribosome profiling is based on the discovery that the mRNA within a ribosome...
easy way to monitor ribosome biogenesis in contrast to laborious polysomeprofiling methods. The fluorescent protein fragments used in BiFC have been...
In 2018, she with the collaboration of Jennifer Van Eyk, developed polysomeprofiling and azidohomoalanine labeling to interrogate the newly-synthesized...
takes place. Ribosome profiling is however still in early stages and has biases that need to be explored further. Ribosome profiling allows for translation...
Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V (2004). "Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs...
transcripts active in transcription (although a more dated method, called polysome fractionation, is still popular in some labs) Protein levels can be analysed...
Sofiadis A, Juhlin CC, et al. (2015). "Differential protein expression profiles of cyst fluid from papillary thyroid carcinoma and benign thyroid lesions"...
Mukherji M, Stettler-Gill M, Peters EC (May 2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized...
and later for his many other researches such as the discovery of "ringed polysome figures" and on theories on gene expression changes with evolution and...
A. (1993-04-01). "Nuclear Mutants of Maize with Defects in Chloroplast Polysome Assembly Have Altered Chloroplast RNA Metabolism". The Plant Cell. 5 (4):...
fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes". Proceedings of the National Academy of Sciences of the United States...
have been seen during development in C.elegans and are associated with polysomes. How this modification is regulated in certain cell types and the exact...
reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein" (PDF). Journal of Cell Biology. 91 (2):...
proteases, is synthesized as a 40-50 kDa proprotein within cytoplasmic polysomes bound to cell membranes. Within the endoplasmic reticulum, these elongated...
(1968). "Control of haemoglobin synthesis: a difference in the size of the polysomes making alpha and beta chains". Nature. 220 (5166): 481–483. Bibcode:1968Natur...
PMID 15489334. Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1):...
giving CAT controlling patents in the field of polysome display. Polysome display involves the use of polysomes, a type of molecule responsible for protein...
1021/bi047328f. PMID 15835887. Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and...
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