In molecular biology, pertactin (PRN) is a highly immunogenic virulence factor of Bordetella pertussis, the bacterium that causes pertussis. Specifically, it is an outer membrane protein that promotes adhesion to tracheal epithelial cells. PRN is purified from Bordetella pertussis and is used for the vaccine production as one of the important components of acellular pertussis vaccine.[2]
A large part of the N-terminus of the pertactin protein is composed of beta helix repeats.[3] This region of the pertactin protein is secreted through the C-terminal autotransporter. The N-terminal signal sequences promotes the secretion of PRN into the periplasm through the bacterial secretion system (Sec) and consequently, the translocation into the outer membrane where it is proteolytically cleaved.[4] The loops in the right handed β-helix of the N-terminus that protrudes out of cell surface (region R1) contains sequence repeats Gly-Gly-Xaa-Xaa-Pro and the RGD domain Arg-Gly-Asp.[4] This RGD domain allows PRN to function as an adhesin and invasin, binding to integrins on the outer membrane of the cell. Another loop of the extending β-helix is region 2 (R2) which contains Pro-Gln-Pro (PQP) repeats towards the C-terminus.[4] This protein’s contribution to immunity is still premature. Reports suggest that R1 and R2 are immunogenic regions, however, recent studies regarding genetic variation of those regions prove otherwise.
^Emsley, P.; Charles, I. G.; Fairweather, N. F.; Isaacs, N. W. (1996). "Structure of Bordetella pertussis virulence factor P.69 pertactin". Nature. 381 (6577): 90–92. Bibcode:1996Natur.381...90E. doi:10.1038/381090a0. PMID 8609998.
^Poolman JT, Hallander HO (February 2007). "Acellular pertussis vaccines and the role of pertactin and fimbriae". Expert Rev Vaccines. 6 (1): 47–56. doi:10.1586/14760584.6.1.47. PMID 17280478.
^ abcInatsuka CS, Xu Q, Vujkovic-Cvijin I, Wong S, Stibitz S, Miller JF, Cotter PA (July 2010). "Pertactin is required for Bordetella species to resist neutrophil-mediated clearance". Infection and Immunity. 78 (7): 2901–9. doi:10.1128/IAI.00188-10. PMC 2897405. PMID 20421378.
In molecular biology, pertactin (PRN) is a highly immunogenic virulence factor of Bordetella pertussis, the bacterium that causes pertussis. Specifically...
series of protein adhesins.. These include filamentous haemaglutinin, pertactin, fimbriae, and pertussis toxin (though expression of pertussis toxin is...
pertussis toxin, adenylate cyclase toxin, filamentous hæmagglutinin, pertactin, fimbria, and tracheal cytotoxin. The bacteria are spread by airborne...
PRN:, a printer device name in DOS Pseudorandom noise, in cryptography Pertactin (PRN), a virulent factor of the bacterium Bordetella pertussis Pro re...
components such as filamentous haemagglutinin, fimbrial antigens and pertactin. Whole-cell (wP) remains the vaccine of choice in low and middle-income...
r., PR per rectum rectally p.r.n., PRN pro re nata as needed PRN is pertactin, a virulence factor of the bacterium that causes pertussis pt. perstetur...
Surface proteins of B. pertussis, including filamentous hemaglutinin and pertactin, mediate attachment to the epithelium. The bacteria then multiply. In...
aP toxoid 3.2 to 40 DTaP aP filamentous agglutinin 2.5 - 34.4 DTaP aP Pertactin 1.6 - 23.4 DTaP aP fimbriae 2 0.8 to 5 DTaP aP fimbriae 3 5 Haemophilus...
colonization by UPEC. Moreover, the Bordetella pertussis adhesins FHA and pertactin are components of three of the four acellular pertussis vaccines currently...
1998). "Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for...
to be dominated by a protein fold known as a beta helix, typified by pertactin. The folding of this domain is thought to be intrinsically linked to its...
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