I. sakaiensis PETase (A0A0K8P6T7) in complex with HEMT, a PET analogue (PDB: 5XH3).
Identifiers
EC no.
3.1.1.101
Alt. names
PET hydrolase, poly(ethylene terephthalate) hydrolase
Databases
IntEnz
IntEnz view
BRENDA
BRENDA entry
ExPASy
NiceZyme view
KEGG
KEGG entry
MetaCyc
metabolic pathway
PRIAM
profile
PDB structures
RCSB PDB PDBe PDBsum
Search
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articles
PubMed
articles
NCBI
proteins
PETases are an esterase class of enzymes that catalyze the breakdown (via hydrolysis) of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl terephthalate (MHET). The idealized chemical reaction is:
where n is the number of monomers in the polymer chain, though a trace amount of the PET breaks down instead to bis(2-hydroxyethyl) terephthalate (BHET).[1] PETases can also break down PEF-plastic (polyethylene-2,5-furandicarboxylate), which is a bioderived PET replacement, into the analogous MHEF. PETases can't catalyze the hydrolysis of aliphatic polyesters like polybutylene succinate or polylactic acid.[2]
Whereas the degradation of PET by natural (non-enzymatic) means will take hundreds of years, PETases can degrade it in a matter of days.[3]
^Cite error: The named reference Yoshida 2016 was invoked but never defined (see the help page).
^Austin HP, Allen MD, Donohoe BS, Rorrer NA, Kearns FL, Silveira RL, et al. (May 2018). "Characterization and engineering of a plastic-degrading aromatic polyesterase". Proceedings of the National Academy of Sciences of the United States of America. 115 (19): E4350–E4357. Bibcode:2018PNAS..115E4350A. doi:10.1073/pnas.1718804115. PMC 5948967. PMID 29666242.
^Dockrill, Peter. "Scientists Have Accidentally Created a Mutant Enzyme That Eats Plastic Waste". ScienceAlert. Retrieved 2018-11-27.
PETases are an esterase class of enzymes that catalyze the breakdown (via hydrolysis) of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl...
bioremediation applications in combination with the PETase enzyme. It degrades the MHET produced by the PETase into ethylene glycol and terephthalic acid. Once...
acid, the PET degradation product by PETase, to ethylene glycol and terephthalic acid. This pair of enzymes, PETase and MHETase, enable the bacterium Ideonella...
PET can be carried out using different enzymes like PETase, cutinase, esterase, lipase, etc. PETase has been also reported for enzymatic degradation of...
initiated by terephthalate 1,2-dioxygenase. Combined with the previously known PETase and MHETase, a full pathway for PET plastic degradation can be engineered...
be a purely chemical process but may also be promoted by enzymes such as PETase. Such technologies have lower energy costs than thermal depolymerisation...
to biodegrade PET. In 2020, the PET degrading enzyme of the bacterium, PETase, has been genetically modified and combined with MHETase to break down PET...
University of South Florida, solved the high-resolution structure of the PETase enzyme using X-ray crystallography at the Diamond Light Source. The team...
in PET can be targeted. A number of enzymes capable of hydrolysing PET (PETases) have been reported. The appeal of enzymatic hydrolysis is that it can...
engineered refinement and mechanical description of synergistic enzymes – PETase, first discovered in 2016, and MHETase of Ideonella sakaiensis – for faster...
engineered refinement and mechanical description of synergistic enzymes – PETase, first discovered in 2016, and MHETase of Ideonella sakaiensis – for faster...
Adhered to a low-grade PET film, the bacteria used two novel enzymes, PETase and MHETase, to decompose the plastic into two environmentally benign substances...
Researchers at the University of Portsmouth have discovered that mixing PETase with a second enzyme called MHETase has created an “enzyme cocktail” that...
engineered refinement and mechanical description of synergistic enzymes – PETase, first discovered in 2016, and MHETase of Ideonella sakaiensis – for faster...
engineered refinement and mechanical description of synergistic enzymes – PETase, first discovered in 2016, and MHETase of Ideonella sakaiensis – for faster...
related to Cutinase. Look up cutinase in Wiktionary, the free dictionary. PETase Longhi S, Czjzek M, Lamzin V, Nicolas A, Cambillau C (May 1997). "Atomic...
closed loop. As demonstrated in the figure, the enzyme groups cellulase, PETase, and keratinase are used within the closed recycling loop to break down...
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