Molecular Inversion Probe (MIP)[1] belongs to the class of Capture by Circularization molecular techniques[1] for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome.[2] Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping[3] as well as for studying gene copy alterations[4]
and characteristics of specific genomic loci[2][5] to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.
^ abAbsalan, Farnaz; Mostafa Ronaghi (2008). Comparative Genomics. Methods in Molecular Biology. Vol. 396. Humana Press. pp. 315–330. doi:10.1007/978-1-59745-515-2. ISBN 978-1-934115-37-4. S2CID 3690014.
^ abCite error: The named reference Turner et al. was invoked but never defined (see the help page).
^Cite error: The named reference 1Hardenbol et al. was invoked but never defined (see the help page).
^Wang Y, Moorhead M, Karlin-Neumann G, Falkowski M, Chen C, Siddiqui F, Davis RW, Willis TD, Faham M (2007). "Direct selection of human genomic loci by microarray hybridization". Nucleic Acids Res. 33 (21): e183. doi:10.1093/nar/gni177. PMC 1301601. PMID 16314297.
^Cite error: The named reference Akhras et al. was invoked but never defined (see the help page).
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