Microfluidic cell culture integrates knowledge from biology, biochemistry, engineering, and physics to develop devices and techniques for culturing, maintaining, analyzing, and experimenting with cells at the microscale.[1][2] It merges microfluidics, a set of technologies used for the manipulation of small fluid volumes (μL, nL, pL) within artificially fabricated microsystems, and cell culture, which involves the maintenance and growth of cells in a controlled laboratory environment.[3][4] Microfluidics has been used for cell biology studies as the dimensions of the microfluidic channels are well suited for the physical scale of cells (in the order of magnitude of 10 micrometers).[2] For example, eukaryotic cells have linear dimensions between 10 and 100 μm which falls within the range of microfluidic dimensions.[4] A key component of microfluidic cell culture is being able to mimic the cell microenvironment which includes soluble factors that regulate cell structure, function, behavior, and growth.[2] Another important component for the devices is the ability to produce stable gradients that are present in vivo as these gradients play a significant role in understanding chemotactic, durotactic, and haptotactic effects on cells.[2]
^ abcdYoung EW, Beebe DJ (March 2010). "Fundamentals of microfluidic cell culture in controlled microenvironments". Chemical Society Reviews. 39 (3): 1036–48. doi:10.1039/b909900j. PMC 2967183. PMID 20179823.
^Mehling M, Tay S (February 2014). "Microfluidic cell culture". Current Opinion in Biotechnology. 25: 95–102. doi:10.1016/j.copbio.2013.10.005. PMID 24484886.
^ abWhitesides GM (July 2006). "The origins and the future of microfluidics". Nature. 442 (7101): 368–73. Bibcode:2006Natur.442..368W. doi:10.1038/nature05058. PMID 16871203. S2CID 205210989.
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Microfluidiccellculture integrates knowledge from biology, biochemistry, engineering, and physics to develop devices and techniques for culturing, maintaining...
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Cellculture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. After...
Human cells have also been manipulated in Digital Microfluidic Immunocytochemistry in Single Cells (DISC) where DMF platforms were used to culture and use...
the widespread use of PDMS in microfluidiccellculture. Like many microfluidic technologies, open system microfluidics has been applied to nanotechnology...
vivo-like cell metabolism and functions study, as well as drug discovery, represents a significant hurdle for microfluidic 3D cellculture devices. Another...
microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic cell sorting...
Microfluidics in chemical biology is the application of microfluidics in the study of chemical biology. Due to its physical dimensions, microfluidics...
experimentation of cellculture processes. Bioprocessors are also used for concentrating bioparticles (such as cells) in bioanalytical systems. Microfluidic processes...
S2CID 205210989. Young EW, Beebe DJ (March 2010). "Fundamentals of microfluidiccellculture in controlled microenvironments". Chemical Society Reviews. 39...
permanently bond PDMS Microfluidic chips with glass slides or PDMS slabs to create water-tight microchannels. Blood plasma separation Single Cell RNA Sequencing...
Hankemeier, Thomas; Vulto, Paul (26 Jul 2013). "Microfluidic titer plate for stratified 3D cellculture". Lab on a Chip. 13 (18): 3548–3554. doi:10.1039/C3LC50210D...
ERC Advanced grant that aimed at developing microfabricated and microfluidiccellculture platforms for improving organoids. The conception of this grant...
PACE project developed the first Omega Machine, a microfluidic life support system for artificial cells that could complement chemically missing functionalities...
(2018). "Investigations into the cancer stem cell niche using in-vitro 3-D tumor models and microfluidics". Biotechnology Advances. 36 (4): 1094–1110....
used in CellSearch or MACS. Other techniques under research include microfluidic separation and combination of immunomagnetic assay and microfluidic separation...
far-western blotting, diffusion blotting, single-cell resolution western blotting, and automated microfluidic western blotting. Researchers use different software...
fabrication techniques developed for microfluidic circuits to RPS sensing. This translation of RPS technology to the microfluidic domain enables very small constrictions...
whereas the aperture format offers a wide variety of aperture sizes. Microfluidic approaches have been used to apply the Coulter principle to lab-on-a-chip...
Robert; Lee, Luke P. (2004). "Continuous perfusion microfluidiccellculture array for high-throughput cell-based assays". Biotechnology and Bioengineering...
more accurate, as well as developing new methods for testing, such as microfluidics. In clinical medicine, antibiotics are most frequently prescribed on...
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