Ribbon schematic of micrococcal nuclease 3D structure, with Ca2+ and TdtP inhibitor
Identifiers
EC no.
3.1.31.1
CAS no.
9013-53-0
Databases
IntEnz
IntEnz view
BRENDA
BRENDA entry
ExPASy
NiceZyme view
KEGG
KEGG entry
MetaCyc
metabolic pathway
PRIAM
profile
PDB structures
RCSB PDB PDBe PDBsum
Search
PMC
articles
PubMed
articles
NCBI
proteins
Thermonuclease
Identifiers
Organism
Staphylococcus aureus
Symbol
nuc
UniProt
P00644
Search for
Structures
Swiss-model
Domains
InterPro
Staphylococcal nuclease
Identifiers
Symbol
?
Pfam
PF00565
Pfam clan
CL0049
InterPro
IPR016071
PROSITE
PDOC00865
CATH
1tt2
SCOP2
1tt2 / SCOPe / SUPFAM
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
and 23 Related for: Micrococcal nuclease information
that have been bound by proteins. MNase-seq (MicrococcalNuclease sequencing) uses the micrococcalnuclease enzyme to identify nucleosome positioning throughout...
3.1.21 through 3.1.25. Examples include: DNA restriction enzymes micrococcalnuclease Ribonuclease UvrABC endonuclease Endodeoxyribonucleases at the U...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well-positioned nucleosomes. Use of the micrococcal nuclease...
Two techniques can localize the ribosomal pause site in vivo; a micrococcalnuclease protection assay and isolation of polysomal transcript. Isolation...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
sonication or treatment with a non-specific restriction enzyme (e.g., micrococcalnuclease). Modification-specific antibodies in turn, are used to immunoprecipitate...
database CUT&RUN sequencing, antibody-targeted controlled cleavage by micrococcalnuclease instead of ChIP, allowing for enhanced signal-to-noise ratio during...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. Micrococcalnuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
linked to cell division. In aging and dividing yeast MNase-seq (MicrococcalNuclease sequencing) showed a loss of nucleosomes of ~50%. Proper histone...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
2. MicrococcalNuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes. Use of the micrococcal nuclease...
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