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Isosbestic point in the bromocresol green spectrum. The spectra of basic, acid and intermediate pH solutions are shown. The analytical concentration of the dye is the same in all solutions.
In spectroscopy, an isosbestic point is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during a chemical reaction or a physical change of the sample. The word derives from two Greek words: "iso", meaning "equal", and "sbestos", meaning "extinguishable".[1]
^IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "isosbestic point". doi:10.1351/goldbook.I03310
In spectroscopy, an isosbesticpoint is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during...
maxima) for the individual species. None of the wavelengths may be an isosbesticpoint for a pair of species. The set of the following simultaneous equations...
characteristic blue-green color. The acid and basic forms of this dye have an isosbesticpoint in their UV-Visible spectrum, around 515 nm, indicate that the two...
the maximum absorption and isosbestic points of the indicator. The isosbesticpoint of GCaMP is the calcium-independent signal, approximately 405-415 nm...
in situ to give oxidized, ring-opened products. Fluorescein has an isosbesticpoint (equal absorption for all pH values) at 460 nm. Many derivatives of...
change from red–violet at 25 °C to pale yellow at 150 °C. An isosbesticpoint (a point where the absorbance curves at all temperatures overlap) indicates...
of linearly polarized light with a wavelength of 550 nanometers, an isosbesticpoint for hemoglobin, thus imaging the erythrocytes as they are flowing through...
densities of a blood sample flowing through small-bore glass tubes at isosbestic wavelengths for deoxyhemoglobin and oxyhemoglobin and the product of the...
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