Oppositely charged particles interact as they are moved through a column. While paired there is more tendency to flow through the column, in reverse phase chromatography.
Ion interaction chromatography (ion-pair chromatography) is a laboratory technique for separating ions with chromatography. In this technique ions are mixed with ion pairing reagents (IPR).[1] The analyte combines with its reciprocal ion in the IPR, this corresponds to retention time. Often organic salts are selected to pair with solute(s). The formation of this pair affects the interaction of the pair with the mobile phase and the stationary phase.[2]
Ioninteractionchromatography (ion-pair chromatography) is a laboratory technique for separating ions with chromatography. In this technique ions are...
Ionchromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity...
Hydrophilic interactionchromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly...
Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the...
column with a targeted affinity. Ion exchange chromatography (usually referred to as ionchromatography) uses an ion exchange mechanism to separate analytes...
of ions that associate with each other, as ion pairs, ion triplets, etc. Ion pairs are also classified according to the nature of the interaction as contact...
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate...
liquid, and supercriticial fluid chromatography. As shown above, the resolving power depends on the total voltage drop the ion traverses. Using a drift voltage...
other tertiary ammonium salts, are used as an ion-interaction reagent in ioninteractionchromatography, due to their amphiphilic properties. Unlike quaternary...
Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without...
in some gas chromatography systems. Chemical ionization (CI) is a lower energy process than electron ionization because it involves ion/molecule reactions...
columns for techniques such as ion exchange, gel filtration (size exclusion), hydrophobic interaction, and affinity chromatography are available. FPLC differs...
gel or an ion exchange column. Second, proteins can be separated according to their size or molecular weight via size exclusion chromatography or by SDS-PAGE...
written as H3O+, the type of oxonium ion produced by protonation of water. It is often viewed as the positive ion present when an Arrhenius acid is dissolved...
included ion exchange chromatography, hydrophobic interactionchromatography, and size-exclusion chromatography (fast protein liquid chromatography), and...
measure the interaction of a material and heat. Separation processes are used to decrease the complexity of material mixtures. Chromatography, electrophoresis...
Denaturing High Performance Liquid Chromatography (DHPLC) is a method of chromatography for the detection of base substitutions, small deletions or insertions...
Chiral column chromatography is a variant of column chromatography that is employed for the separation of chiral compounds, i.e. enantiomers, in mixtures...
creating ions. Electrospray is used to study protein folding. Electrospray ionization is the ion source of choice to couple liquid chromatography with mass...
"Performance of charged aerosol detection with hydrophilic interactionchromatography". Journal of Chromatography A. 1405: 72–84. doi:10.1016/j.chroma.2015.05.050...
and Ion Physics. 46: 399–402. Bibcode:1983IJMSI..46..399K. doi:10.1016/0020-7381(83)80136-3. James M. Miller (16 December 2005). Chromatography: Concepts...
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