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Huh7 is an immortalised cell line and may be grown in the laboratory for research purposes. According to the web site huh7.com, it is "a well differentiated hepatocyte-derived carcinoma cell line, originally taken from a liver tumor in a 57-year-old Japanese male in 1982."[1] It is used extensively in hepatitis C and dengue virus research.[2][3]
Huh7 cells have been instrumental in hepatitis C research. Until 2005, it was not possible to culture hepatitis C in the laboratory. The introduction of the Huh7 cell line permitted screening of drug candidates against laboratory-cultured hepatitis C virus and permitted the development of new drugs against hepatitis C.[4][2][5]
In February 2022 it was reported that the Huh7 cells can reverse transcribe at least parts of the code of the mRNA from the BNT162b2 vaccine into DNA (the study doesn't prove that that DNA is integrated).[6]
^"HuH-7 Cell Line". Retrieved 2016-07-05.
^ abSainz, Bruno; Tencate, Veronica; Uprichard, Susan L (2009). "Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection". Virology Journal. 6: 103. doi:10.1186/1743-422X-6-103. PMC 2719612. PMID 19604376.
^Behnam, Mira A. M.; Nitsche, Christoph; Boldescu, Veaceslav; Klein, Christian D. (2016). "The medicinal chemistry of dengue virus". Journal of Medicinal Chemistry. 59 (12): 5622–5649. doi:10.1021/acs.jmedchem.5b01653. PMID 26771861.
^Bartenschlager, R; Pietschmann, T (2005). "Efficient hepatitis C virus cell culture system: what a difference the host cell makes". Proceedings of the National Academy of Sciences of the United States of America. 102 (28): 9739–40. Bibcode:2005PNAS..102.9739B. doi:10.1073/pnas.0504296102. PMC 1175013. PMID 15998731.
^Meier, Volker; Ramadori, Giuliano (2009). "Hepatitis C virus virology and new treatment targets". Expert Review of Anti-infective Therapy. 7 (3): 329–50. doi:10.1586/eri.09.12. PMID 19344246. S2CID 38411966.
^Aldén, Markus; Olofsson Falla, Francisko; Yang, Daowei; Barghouth, Mohammad; Luan, Cheng; Rasmussen, Magnus; De Marinis, Yang (25 February 2022). "Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line". Current Issues in Molecular Biology. 44 (3): 1115–1126. doi:10.3390/cimb44030073. PMC 8946961. PMID 35723296.
Huh7 is an immortalised cell line and may be grown in the laboratory for research purposes. According to the web site huh7.com, it is "a well differentiated...
of lipid metabolism reduces/removes lipid droplets from HuH7 cells. In hepatitis C–infected HuH7 cells, this reduction/removal of lipid droplets by triacsin...
patient Henrietta Lacks. HEK 293 cells – derived from human fetal cells. Huh7 cells – hepatocyte-derived carcinoma cell line Jurkat cells – a human T lymphocyte...
Japanese team was able to propagate a molecular clone in a cell culture called Huh7. This discovery enabled proper characterization of the viral particle and...
Acid Inhibits Growth and Metastatic Potential in Liver Cancer HepG2 and Huh7 Cells". Biological and Pharmaceutical Bulletin. 46 (1): 35–41. doi:10.1248/bpb...
be responsible for potential endogenisation of the SARS-CoV-2 genome in Huh7 mutant cancer cells, which would possibly explain why some patients test...
mouse RAW264.7 macrophages, macrophages differentiated from human monocytes, Huh7 human liver carcinoma cells, human MCF-7 breast cancer cells, human A549...
Cell line Origin CaCo2 human colon carcinoma Hep G2 human liver carcinoma Huh7 human well differentiated hepatocyte-derived carcinoma PLC/PRF/5 human hepatoma...
capable of reducing tumor volume by suppressing the cell viability of HLE, HuH7, and HuH6 cells, and it has shown effective inhibition of S/G2/M transition...
which the GT-1b replicon system was implemented in Huh7 cells and bovine viral diarrhea virus also in Huh7 cells was used as a counterscreen for specificity...
suppressive properties in human hepatocellular carcinoma-derived cells, Huh7 and Hep3B cell lines, restricting spheroid formation and clonogenic capacity...
encapsulated in SNALPs significantly inhibited hepatic tumor growth in the Huh7-luc+ orthotopic xenograft model of human liver cancer. SiRNA-mediated CSN5...
lactiflora, an Asian flower plant, induces GNMT mRNA and protein expression in Huh7 human hepatoma cells. GRCh38: Ensembl release 89: ENSG00000124713 – Ensembl...
RsYN2012 and RsYN2016 and one clade 2 virus (not using ACE2) RsHuB2019 in Huh7 cells with trypsin. pangolin/Guangdong, in Vero cells, human organoids, hACE2...
liposarcoma cells, MDA-MB-231, MDA-MB-436, and MCF7 breast cancer cells, Huh7 and JHH-4 liver cancer cells, THP-1 acute myeloid leukemia cells, U937 acute...
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