Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references
Chemical compound
Haematoxylin or hematoxylin (/ˌhiːməˈtɒksɪlɪn/), also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree (Haematoxylum campechianum)[1][2] with a chemical formula of C 16H 14O 6. This naturally derived dye has been used as a histologic stain, as an ink[3][4][5][6] and as a dye in the textile and leather industry.[7][8] As a dye, haematoxylin has been called palo de Campeche,[8] logwood extract,[7] bluewood[9] and blackwood.[9] In histology, haematoxylin staining is commonly followed by counterstaining with eosin.[1][10][11] When paired, this staining procedure is known as H&E staining and is one of the most commonly used combinations in histology.[1][7][12][13][14] In addition to its use in the H&E stain, haematoxylin is also a component of the Papanicolaou stain (or Pap stain) which is widely used in the study of cytology specimens.[1][14]
Although the stain is commonly called haematoxylin, the active colourant is the oxidized form haematein, which forms strongly coloured complexes with certain metal ions (commonly Fe(III) and Al(III) salts).[1][7][8][15][16] In its pure form, haematoxylin is a colourless and crystalline solid,[7][17] although commercial samples are typically light to dark brown based on the level of impurities present.[2][18]
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Haematoxylin or hematoxylin (/ˌhiːməˈtɒksɪlɪn/), also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree...
as methylene blue. Haematoxylin and eosin staining is frequently used in histology to examine thin tissue sections. Haematoxylin stains cell nuclei blue...
H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Tissue stained with haematoxylin and eosin...
histological techniques. The standard stains are haematoxylin and eosin, but many others exist. The use of haematoxylin and eosin-stained slides to provide specific...
histology as a component for staining of cell specimens, often together with haematoxylin as PTAH. It binds to fibrin, collagen, and fibres of connective tissues...
section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures...
used in diagnostic histology of animal tissues is Harris' haematoxylin as part of a haematoxylin and eosin (H&E) stain. IUPAC, Compendium of Chemical Terminology...
the cells lining the walls of the crypts) are stained blue-gray with haematoxylin. As seen in panels C and D, crypts are about 75 to about 110 cells long...
is used to produce red and purple dyes. The histological stain called haematoxylin is produced from this species. The wood of the Brazilwood tree (Caesalpinia...
of the substantia nigra are visible in the background. Stains used: mouse monoclonal alpha-synuclein antibody; counterstained with Mayer's haematoxylin...
guanine crystals with lattice parameter indicated with black arrows. (c) Haematoxylin and eosin staining of a cross-section of white skin showing the epidermis...
Hemipelvectomy for gas gangrene Muscle biopsy examined under the microscope (haematoxylin-eosin stain, zoom 100×): the large white areas between the muscle fibers...
of a human proliferating mammary gland during estrous cycle. Sprouting gland tissue can be seen in the upper left field (haematoxylin eosin staining)...
source of dye. The woodchips are still used as an important source of haematoxylin, which is used in histology for staining. The bark and leaves are also...
red inks. It is commonly used in histology, most notably in the H&E (Haematoxylin and Eosin) stain. Eosin Y is also widely used in the Papanicolaou stain...
very metabolically active. Basophilic dyes such as aniline or (weakly) haematoxylin highlight negatively charged components, and so bind to the phosphate...
stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. In Gram staining, crystal violet stains only Gram-positive...
elastic fibers require special staining since when it is stained using haematoxylin and eosin (H&E) stain it appears the same as hyaline cartilage. Verhoeff...
indicative of the presence of lipid dissolved during the staining process. Haematoxylin and eosin stain Specialty Orthopedics, traumatology, pulmonology, intensive...
tracers Methylene blue, blue thiazine dye also used as a medication Haematoxylin, natural stain derived from hearthwood and used in histology Laser dyes...
Hematein (US spelling) or haematein is an oxidized derivative of haematoxylin, used in staining. Haematein should not be confused with haematin, which...
fibers that form tangled masses and appear basophilic under routine haematoxylin and eosin staining. These fibers stain black with the Verhoeff stain...