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Regulatory protein GAL4
Identifiers
Organism
Saccharomyces cerevisiae
Symbol
GAL4
Entrez
855828
UniProt
P04386
Search for
Structures
Swiss-model
Domains
InterPro
The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes.[1] This protein represents a large fungal family of transcription factors, Gal4 family, which includes over 50 members in the yeast Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3.[2]
Gal4 recognizes genes with UASG, an upstream activating sequence, and activates them. In yeast cells, the principal targets are GAL1 (galactokinase), GAL10 (UDP-glucose 4-epimerase), and GAL7 (galactose-1-phosphate uridylyltransferase), three enzymes required for galactose metabolism. This binding has also proven useful in constructing the GAL4/UAS system, a technique for controlling expression in insects.[3] In yeast, Gal4 is by default repressed by Gal80, and activated in the presence of galactose as Gal3 binds away Gal80.[4]
^Klar AJ, Halvorson HO (1974). "Studies on the positive regulatory gene, GAL4, in regulation of galactose catabolic enzymes in Saccharomyces cerevisiae". Molecular & General Genetics. 135 (3): 203–12. doi:10.1007/BF00268616. PMID 4376212. S2CID 26014344.
^Schjerling P, Holmberg S (December 1996). "Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators". Nucleic Acids Research. 24 (23): 4599–607. doi:10.1093/nar/24.23.4599. PMC 146297. PMID 8967907.
^Duffy JB (2002). "GAL4 system in Drosophila: a fly geneticist's Swiss army knife". Genesis. 34 (1–2): 1–15. doi:10.1002/gene.10150. PMID 12324939. S2CID 5073328.
^Cite error: The named reference pmid19651897 was invoked but never defined (see the help page).
and 22 Related for: Gal4 transcription factor information
the Gal4transcriptionfactor is mediated by acidic amino acids, whereas hydrophobic residues in Gcn4 play a similar role. Hence, the TADs in Gal4 and...
a functional homolog of Pdr1p. Pdr1p and Pdr3p is a part of Gal4transcriptionfactor family due to their zinc-finger DNA binding motif, which is located...
containing transcriptionfactors (fork head box (FOX) and NF-Y) and other transcriptionfactors that use zinc finger(s) for DNA binding (Groucho TLE, Gal4, and...
(February 2012). "Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4". Proc. Natl. Acad. Sci...
activate the transcription of the GAL1/GAL7/GAL10 cassette. On the other hand, a MIG1 response to the presence of glucose can inhibit GAL4 and therefore...
released from the complex and transcription begins. The property of the GAL1-GAL10 to bind the GAL4 protein is utilised in the GAL4/UAS technique for controlled...
a characteristic motif of transcriptionfactors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression...
residues. These zinc fingers can be found in several transcriptionfactors including the yeast Gal4 protein. The zinc finger antiviral protein (ZAP) binds...
modes of transcriptional control in yeast. One important study of this topic was conducted using the Gal4-VP16 artificial transcriptionfactor system,...
engineer pdfr-GAL4 lines to show that Gal4 mediated rescue of pdfr phenotypes is insufficient to provide complete behavioral rescue. A series of GAL4 driver...
interactions. This system is based on a transcriptionfactor, originally GAL4, whose separate DNA-binding and transcription activation domains are both required...
Common techniques such as overexpressing or misexpressing the genes by UAS-Gal4 system in D. melanogaster are used. In model organisms, such techniques are...
essential factor for Gal4 dependent transactivation by T.Fukasawa and F.Winston labs. Transcriptionfactors Gcn4, Pho4, Msn2, Ino2, members of the Gal4 family...
disciplines may target specific cell types and tag them for degradation. The GAL4/UAS System "reports" a subset of genes of interest although it has no independent...
as how the yeast transcriptional activator Gal4 works. He was the originator of the "ball and stick" model of transcriptionfactor function, demonstrating...
were expressed. They used the GAL4/UAS system to examine how the expression of certain clock genes, pigment dispersing factor gene (pdf) and long and short...
created fusion proteins that used LexA to bring portions of yeast Gal4 and other transcription regulatory proteins to synthetic reporter genes in yeast. These...
offer the option of studying transcriptional activators: in these experiments typically the GAL4/UAS_system is used and its Gal4 upstream activating DNA sequence...
biotrophic and necrotrophic phases. The gene CLTA1 encodes a GAL4‐like transcriptional activator, which is consistent with a role in reprogramming metabolism...
reported transcriptionfactor. 9aaTAD is a novel domain common to a large superfamily of eukaryotic transcriptionfactors represented by Gal4, Oaf1, Leu3...
domain of the transcriptionfactorGAL4 against potential interacting proteins or a cDNA library that express , for example, the GAL4 activation domain...
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