the crystal structure of a binary u5 snrnp complex
Identifiers
Symbol
GYF
Pfam
PF02213
InterPro
IPR003169
SMART
GYF
SCOP2
1gyf / SCOPe / SUPFAM
CDD
cd00072
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
In molecular biology, the GYF domain (glycine-tyrosine-phenylalanine domain) is an approximately 60-amino acid protein domain which contains a conserved GP[YF]xxxx[MV]xxWxxx[GN]YF motif. It was identified in the human intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two tandem PPPGHR segments within the cytoplasmic region of CD2. Binding experiments and mutational analyses have demonstrated the critical importance of the GYF tripeptide in ligand binding. A GYF domain is also found in several other eukaryotic proteins of unknown function.[1] It has been proposed that the GYF domain found in these proteins could also be involved in proline-rich sequence recognition.[2] Resolution of the structure of the CD2BP2 GYF domain by NMR spectroscopy revealed a compact domain with a beta-beta-alpha-beta-beta topology, where the single alpha-helix is tilted away from the twisted, anti-parallel beta-sheet. The conserved residues of the GYF domain create a contiguous patch of predominantly hydrophobic nature which forms an integral part of the ligand-binding site.[2] There is limited homology within the C-terminal 20-30 amino acids of various GYF domains, supporting the idea that this part of the domain is structurally but not functionally important.[3]
^Nishizawa K, Freund C, Li J, Wagner G, Reinherz EL (December 1998). "Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation". Proc. Natl. Acad. Sci. U.S.A. 95 (25): 14897–902. Bibcode:1998PNAS...9514897N. doi:10.1073/pnas.95.25.14897. PMC 24547. PMID 9843987.
^ abFreund C, Dotsch V, Nishizawa K, Reinherz EL, Wagner G (July 1999). "The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences". Nat. Struct. Biol. 6 (7): 656–60. doi:10.1038/10712. PMID 10404223. S2CID 19688996.
^Freund C, Kuhne R, Yang H, Park S, Reinherz EL, Wagner G (November 2002). "Dynamic interaction of CD2 with the GYF and the SH3 domain of compartmentalized effector molecules". EMBO J. 21 (22): 5985–95. doi:10.1093/emboj/cdf602. PMC 137194. PMID 12426371.
In molecular biology, the GYFdomain (glycine-tyrosine-phenylalanine domain) is an approximately 60-amino acid protein domain which contains a conserved...
encoding protein Transmembrane protein 182 TNRC15: PERQ amino acid-rich with GYFdomain-containing protein 2 TSGA10 encoding protein Testis specific 10 TTN: titin...
Kofler M, Heuer K, Zech T, Freund C (2004). "Recognition sequences for the GYFdomain reveal a possible spliceosomal function of CD2BP2". J. Biol. Chem. 279...
PERQ amino acid-rich with GYFdomain-containing protein 2 is a protein that in humans is encoded by the GIGYF2 gene. GRCh38: Ensembl release 89: ENSG00000204120...
SOLUTION STRUCTURES OF THE BRK DOMAINS OF THE HUMAN CHROMO HELICASE DOMAIN 7 AND 8, REVEALS STRUCTURAL SIMILARITY WITH GYFDOMAIN SUGGESTING A ROLE IN PROTEIN...
Freund C (September 2005). "Novel interaction partners of the CD2BP2-GYFdomain". The Journal of Biological Chemistry. 280 (39): 33397–402. doi:10.1074/jbc...
PMID 17081983. S2CID 7827573. Kofler M, Motzny K, Freund C (2006). "GYFdomain proteomics reveals interaction sites in known and novel target proteins"...
proliferation, differentiation, and apoptosis. This protein and Grb10-interacting GYF protein 2 have been identified as a components of the mammalian 4EHP (m4EHP)...
"Recruitment of the RNA helicase RHAU to stress granules via a unique RNA-binding domain". The Journal of Biological Chemistry. 283 (50): 35186–35198. doi:10.1074/jbc...
Governor of Berwick: Albeit I be na gud seeman, I promes unto your lordschip, gyf I may anes enconter with hym eyther be see or land, he sall eyther carre...