Predicted secondary structure and sequence conservation of DsrA
Identifiers
Symbol
DsrA
Rfam
RF00014
Other data
RNA type
Gene; sRNA
Domain(s)
Bacteria
SO
SO:0000378
PDB structures
PDBe
DsrA RNA is a non-coding RNA that regulates both transcription, by overcoming transcriptional silencing by the nucleoid-associated H-NS protein,[1] and translation, by promoting efficient translation of the stress sigma factor, RpoS.[2][3] These two activities of DsrA can be separated by mutation: the first of three stem-loops of the 85 nucleotide RNA is necessary for RpoS translation but not for anti-H-NS action, while the second stem-loop is essential for antisilencing and less critical for RpoS translation. The third stem-loop, which behaves as a transcription terminator, can be substituted by the trp transcription terminator without loss of either DsrA function. The sequence of the first stem-loop of DsrA is complementary with the upstream leader portion of RpoS messenger RNA, suggesting that pairing of DsrA with the RpoS message might be important for translational regulation. The structures of DsrA and DsrA/rpoS complex were studied by NMR. The study concluded that the sRNA contains a dynamic conformational equilibrium for its second stem–loop which might be an important mechanism for DsrA to regulate the translations of its multiple target mRNAs.[4]
There is evidence that DsrA RNA can self-assemble into nanostructures through antisense interactions of three self-complementary regions.[5][6]
^Sledjeski, D; Gottesman S (1995). "A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli". Proc Natl Acad Sci USA. 92 (6): 2003–2007. Bibcode:1995PNAS...92.2003S. doi:10.1073/pnas.92.6.2003. PMC 42411. PMID 7534408.
^Majdalani, N; Cunning C; Sledjeski D; Elliott T; Gottesman S (1998). "DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription". Proc Natl Acad Sci USA. 95 (21): 12462–12467. Bibcode:1998PNAS...9512462M. doi:10.1073/pnas.95.21.12462. PMC 22853. PMID 9770508.
^Gottesman, S (2005). "Micros for microbes: non-coding regulatory RNAs in bacteria". Trends Genet. 21 (7): 399–404. CiteSeerX 10.1.1.391.8944. doi:10.1016/j.tig.2005.05.008. PMID 15913835.
^Wu, Pengzhi; Liu, Xiaodan; Yang, Lingna; Sun, Yitong; Gong, Qingguo; Wu, Jihui; Shi, Yunyu (2017-09-19). "The important conformational plasticity of DsrA sRNA for adapting multiple target regulation". Nucleic Acids Research. 45 (16): 9625–9639. doi:10.1093/nar/gkx570. ISSN 1362-4962. PMC 5766208. PMID 28934467.
^Cayrol B, Nogues C, Dawid A, Sagi I, Silberzan P, Isambert H (2009). "A Nanostructure Made of a Bacterial Noncoding RNA". J Am Chem Soc. 131 (47): 17270–17276. doi:10.1021/ja906076e. PMID 19821568.
^Cayrol B, Geinguenaud F, Lacoste J, Busi F, Le Dérout J, Piétrement O, Le Cam E, Régnier P, Lavelle C, Arluison V (2009). "Auto-assembly of E. coli DsrA small noncoding RNA: Molecular characteristics and functional consequences." RNA Biol. 6 (4): 434–445. doi:10.4161/rna.6.4.8949. PMID 19535898.
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involved in cell adaptation. At least three sRNAs, DsrA, RprA and OxyS, regulate the translation of RpoS. DsrA and RprA both activate RpoS translation by...
regulator RNA A) increased the translation of RpoS. As with DsrA, RprA is predicted to form three stem-loops. Thus, at least two small RNAs, DsrA and RprA...
rpoS mRNA structure for optimal translation. Hfq activates both DsrA and RprA. In contrast, LeuO inhibits rpoS translation by repressing dsrA expression...
Zhang A (March 2001). "Hfq is necessary for regulation by the untranslated RNADsrA". Journal of Bacteriology. 183 (6): 1997–2005. doi:10.1128/JB.183.6.1997-2005...
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