Global Information Lookup Global Information

Deoxyribozyme information


Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a specific chemical reaction, often but not always catalytic. This is similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).[1] However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,[2][3] there is only little evidence for naturally occurring deoxyribozymes.[4][5] Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.

With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.[6]

In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst;[6] though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix.[1] However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.[1]

In 2021, the DNAmoreDB database for cataloguing known deoxyribozymes was released.[7]

  1. ^ a b c Breaker RR (May 1997). "DNA enzymes". Nature Biotechnology. 15 (5): 427–431. doi:10.1038/nbt0597-427. PMID 9131619. S2CID 1918660.
  2. ^ Kruger K, Grabowski PJ, Zaug AJ, Sands J, Gottschling DE, Cech TR (November 1982). "Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena". Cell. 31 (1): 147–157. doi:10.1016/0092-8674(82)90414-7. PMID 6297745. S2CID 14787080.
  3. ^ Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S (December 1983). "The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme". Cell. 35 (3 Pt 2): 849–857. doi:10.1016/0092-8674(83)90117-4. PMID 6197186. S2CID 39111511.
  4. ^ Köhler T, Patsis PA, Hahn D, Ruland A, Naas C, Müller M, Thiele J (April 2020). "DNAzymes as Catalysts for l-Tyrosine and Amyloid β Oxidation". ACS Omega. 5 (13): 7059–7064. doi:10.1021/acsomega.9b02645. PMC 7143405. PMID 32280846.
  5. ^ Breaker RR, Joyce GF (September 2014). "The expanding view of RNA and DNA function". Chemistry & Biology. 21 (9): 1059–1065. doi:10.1016/j.chembiol.2014.07.008. PMC 4171699. PMID 25237854.
  6. ^ a b Silverman SK (October 2004). "Deoxyribozymes: DNA catalysts for bioorganic chemistry". Organic & Biomolecular Chemistry. 2 (19): 2701–2706. CiteSeerX 10.1.1.626.8241. doi:10.1039/B411910J. PMID 15455136.
  7. ^ Ponce-Salvatierra A, Boccaletto P, Bujnicki JM (January 2021). "DNAmoreDB, a database of DNAzymes". Nucleic Acids Research. 49 (D1): D76–D81. doi:10.1093/nar/gkaa867. PMC 7778931. PMID 33053178.

and 19 Related for: Deoxyribozyme information

Request time (Page generated in 0.5254 seconds.)

Deoxyribozyme

Last Update:

Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a specific chemical reaction...

Word Count : 4795

DNA computing

Last Update:

applications such as preventing hormonal imbalance. Catalytic DNA (deoxyribozyme or DNAzyme) catalyze a reaction when interacting with the appropriate...

Word Count : 4916

Metalloprotein

Last Update:

subunit of ribosomes. Several classes of ribozymes have been described. Deoxyribozymes, also called DNAzymes or catalytic DNA, are artificial DNA-based catalysts...

Word Count : 5303

Logic gate

Last Update:

Stojanovic, Milan N.; Mitchell, Tiffany E.; Stefanovic, Darko (2002). "Deoxyribozyme-Based Logic Gates". Journal of the American Chemical Society. 124 (14):...

Word Count : 3459

DNA

Last Update:

are new methods to test paternity while a mother is still pregnant. Deoxyribozymes, also called DNAzymes or catalytic DNA, were first discovered in 1994...

Word Count : 17856

Catalysis

Last Update:

also exhibit catalytic properties including ribozymes, and synthetic deoxyribozymes. Biocatalysts can be thought of as an intermediate between homogeneous...

Word Count : 5114

Gerald Joyce

Last Update:

work on in vitro evolution, for the discovery of the first DNA enzyme (deoxyribozyme), for his work in discovering potential RNA world ribozymes, and more...

Word Count : 772

Ribozyme

Last Update:

sister ribozyme VS ribozyme Pistol ribozyme Hatchet ribozyme Viroids Deoxyribozyme Spiegelman Monster Catalysis Enzyme RNA world hypothesis Peptide nucleic...

Word Count : 5174

Aptamer

Last Update:

aptamers – Oligonucleotides which recognize the exosites of human thrombin Deoxyribozyme – DNA oligonucleotides that can perform a specific chemical reaction...

Word Count : 6560

RNA world

Last Update:

mRNA was subjected to test-tube evolution to derive a catalytic DNA (Deoxyribozyme, also called DNAzyme) with RNA-cleavage activity. After only a few weeks...

Word Count : 10688

Nucleic acid analogue

Last Update:

non-natural aminoacids for an expanded genetic code. Biotin Dark quencher Deoxyribozyme Expanded genetic code Fluorophore Genetics Molecular biology Nucleic...

Word Count : 5268

Ronald Breaker

Last Update:

with Gerald Joyce. While at Scripps, he isolated the first DNA enzyme (deoxyribozyme). He joined the molecular, cellular, and developmental biology department...

Word Count : 412

Systematic evolution of ligands by exponential enrichment

Last Update:

Aptamer – Oligonucleotide or peptide molecules that bind specific targets Deoxyribozyme – DNA oligonucleotides that can perform a specific chemical reaction...

Word Count : 4741

XYLT1

Last Update:

Buchser WJ, Hurtado A, Grimpe B. (2012) Systemic administration of a deoxyribozyme to xylosyltransferase-1 mRNA promotes recovery after a spinal cord injury...

Word Count : 1531

Locked nucleic acid

Last Update:

Erdmann, Volker A; Kurreck, Jens (May 2004). "Gaining Target Access for Deoxyribozymes". Journal of Molecular Biology. 339 (2): 355–363. doi:10.1016/j.jmb...

Word Count : 1647

DNA nanotechnology

Last Update:

structures and devices can be made using functional nucleic acids such as deoxyribozymes and ribozymes, which can perform chemical reactions, and aptamers, which...

Word Count : 9648

Small molecule sensors

Last Update:

mercury. Chelation Luminescence Fluorescence Fluorophore Biosensor Deoxyribozyme Tomat, Elisa; Lippard, Stephen J (April 2010). "Imaging mobile zinc...

Word Count : 2005

GATA3

Last Update:

study of individuals suffering allergen-induced asthma, inhalation of Deoxyribozyme ST010, which specifically inactivates GATA3 messenger RNA, for 28 days...

Word Count : 3740

Alison Todd

Last Update:

Research (JJR), we were already exploring ways to exploit DNAzymes (deoxyribozymes) for diagnostic applications. These fascinating molecules are simple...

Word Count : 1018

PDF Search Engine © AllGlobal.net