Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells), other cell types that are found in solid tissue require specific techniques to separate them into individual cells. This may be performed by using enzymes to digest the proteins that binds these cells together within the extracellular matrix. After the matrix proteins have been digested, cells remain loosely bound together but can be gently separated mechanically. Following isolation, experiments can be performed on these single isolated cells including patch clamp electrophysiology, calcium fluorescence imaging, and immunocytochemistry.
Cellisolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally...
was originally derived by single-cellisolation from the kidneys of five unsexed, 1-day-old hamsters. BHK-21 cells are susceptible to human adenovirus...
for cellisolation. Cell-types often of interest to purify may be specific leukocytes, such as CD4+ T cells, stem cells, or circulating tumor cells (CTCs)...
fragments. After isolation from septal cartilage biopsies, NC can be extensively expanded in numbers by conventional in vitro cell culture methods (monolayer...
Isolation amplifiers are a form of differential amplifier that allow measurement of small signals in the presence of a high common mode voltage by providing...
Social isolation is a state of complete or near-complete lack of contact between an individual and society. It differs from loneliness, which reflects...
(March 2015). "Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cellisolation". Lab on a Chip. 15 (5):...
process of isolation, losing its stem cell characteristics. Despite 160 years of biological effort to isolate and retrieve plant stem cells, none succeeded...
as the entire process from early cellisolation and cultivation, to cell banking and culture expansion of the cells until final harvest (termination of...
allows the reversible isolation and staining of antigen-specific T-cells. This technology combines a current T-cellisolation method with the Strep-Tag...
sickle cell disease, intimate relationships with all members of the family are avoided. The stigmatization and social isolation people with sickle cell disease...
survival for patients receiving bone marrow or stem cell grafts. When a person is in protective isolation, the room should be properly cleaned and ventilated...
"Cell-type-specific profiling of gene expression and chromatin binding without cellisolation: assaying RNA Pol II occupancy in neural stem cells". Developmental...
variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give...
The mechanisms of reproductive isolation are a collection of evolutionary mechanisms, behaviors and physiological processes critical for speciation. They...
includes cellisolation, which can be performed using low- and high-throughput techniques. This is followed by a qPCR step and then single-cell RNAseq where...
as GAM method. This non-contact laser microdissection system makes cellisolation for further genetic analysis possible. It is the first developed laser...
Some of those cells are reserved for "isolation", where a convict is kept alone in a cell as punishment method. Some isolationcells contain no furnishing...
Cell therapy (also called cellular therapy, cell transplantation, or cytotherapy) is a therapy in which viable cells are injected, grafted or implanted...
Maryland, Todaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. The '3T3' designation...
for cell selection from whole blood or other single cell suspensions. IBA has developed an affinity chromatography system for non-magnetic isolation of...
torture technique aimed at complete sensory deprivation and isolation. A prisoner is held in a cell that deprives them of all senses and identity. It is particularly...
also inhibits some proteases. It is a useful additive for protein or cellisolation procedure. Diisopropyl fluorophosphate (DFP) was a nerve gas developed...