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Buoyant density centrifugation information


Electron microscope image of canine parvovirus isolated using buoyant density centrifugation

Buoyant density centrifugation (also isopycnic centrifugation or equilibrium density-gradient centrifugation) uses the concept of buoyancy to separate molecules in solution by their differences in density.

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Buoyant density centrifugation

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Buoyant density centrifugation (also isopycnic centrifugation or equilibrium density-gradient centrifugation) uses the concept of buoyancy to separate...

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Differential centrifugation

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biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles...

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Virology

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centrifugation, called buoyant density centrifugation, is used. In this method the viruses recovered from differential centrifugation are centrifuged...

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Satellite DNA

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chloride density gradient using buoyant density centrifugation. Sequences with a greater ratio of A+T display a lower density while those with a greater ratio...

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Relative density

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these figures are approximate.) Substances with a relative density of 1 are neutrally buoyant, those with RD greater than one are denser than water, and...

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Ultracentrifuge

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ultracentrifugation Gas centrifuge Theodor Svedberg Differential centrifugation Buoyant density ultracentrifugation Zippe-type centrifuge "Optima MAX-XP". Retrieved...

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Analytical ultracentrifugation

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Ultracentrifuge Gas centrifuge Theodor Svedberg Differential centrifugation Buoyant density ultracentrifugation Zippe-type centrifuge "Technical Manual...

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Protein purification

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point in the vessel where their buoyant density is balanced with centrifugal force. Such an "equilibrium" centrifugation can allow extensive purification...

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Elutriation

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Stokes' law if the particle's Reynolds number is below 0.2. Counterflow centrifugation elutriation is a related technique to separate cells. An air elutriator...

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Minisatellite

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sheared DNA that showed a distinct buoyant density, detectable as a "satellite peak" in density gradient centrifugation, and that was subsequently identified...

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Cyclonic separation

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surrounding fluid. Using ρ f {\displaystyle \rho _{f}} for the density of the fluid, the buoyant force is: F b = − V p ρ f V t 2 r {\displaystyle F_{b}=-V_{p}\rho...

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Sedimentation equilibrium

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reference point z = 0 {\displaystyle z=0} , m ∗ {\displaystyle m^{*}} is the buoyant mass of the colloidal particles, g {\displaystyle g} is the standard acceleration...

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List of measuring instruments

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force field of a centrifuge, substances of different densities separate. pH meter Saturated calomel electrode Hygrometer the density of water in air Lysimeter...

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Matthew Meselson

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separates macromolecules according to their buoyant density. The method, equilibrium density gradient centrifugation, was sufficiently sensitive that Meselson...

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Viscometer

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settling velocity, is reached when this frictional force combined with the buoyant force exactly balance the gravitational force. The resulting settling velocity...

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Centrifugal force

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perpendicular to the axis of rotation, giving rise to large buoyant forces which push low-density particles inward. Elements or particles denser than the...

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Diagnostic microbiology

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ISSN 0893-8512. PMC 358210. PMID 1747860. Carr, Dr Steven M. "CsCl density-gradient centrifugation". www.mun.ca. Retrieved 2017-04-03. Martinez, Lluis. "Magnetic...

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Coconut

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a protein-rich powder, can be processed from coconut milk following centrifugation, separation, and spray drying. Coconut milk and coconut cream extracted...

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Field flow fractionation

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from the techniques capability for high resolution given sufficient buoyant density. This allows for the separation of particles with only a 5% difference...

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Soil mechanics

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{M_{s}}{V_{t}}}} Buoyant Density, ρ ′ {\displaystyle \rho '} , defined as the density of the mixture minus the density of water is useful if the...

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StarTram

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niobium-titanium superconductor is technically sufficient (a critical current density of 5 x 105 A/cm2 under the relevant magnetic field conditions for the levitated...

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Spacetime

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of the liquids trichloroethylene and dibromoethane having the same buoyant density as the Teflon (Fig. 5-9b). Fluorine has atomic number Z = 9, while...

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Ion vibration current

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phenomenon that occurs in a centrifuge, or more simply, it is essentially the same phenomenon that occurs when low-density objects float to the top of...

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Ungulate protoparvovirus 1

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4 × 106 (i.e., about 26.5% of the weight of the complete virion). Buoyant densities (g/mL in cesium chloride) of complete infectious virions, incomplete...

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History of ice drilling

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water that they melt. He added a long section above the hotpoint that was buoyant in wanter, providing a force towards the vertical whenever the drill was...

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