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Bioorthogonal chemical reporter information


In chemical biology, bioorthogonal chemical reporter is a non-native chemical functionality that is introduced into the naturally occurring biomolecules of a living system, generally through metabolic or protein engineering. These functional groups are subsequently utilized for tagging and visualizing biomolecules. Jennifer Prescher and Carolyn R. Bertozzi, the developers of bioorthogonal chemistry, defined bioorthogonal chemical reporters as "non-native, non-perturbing chemical handles that can be modified in living systems through highly selective reactions with exogenously delivered probes." It has been used to enrich proteins and to conduct proteomic analysis.

In the early development of the technique, chemical motifs have to fulfill criteria of biocompatibility and selective reactivity in order to qualify as bioorthogonal chemical reporters. Some combinations of proteinogenic amino acid side chains meet the criteria, as do ketone and aldehyde tags. Azides and alkynes are other examples of chemical reporters.

A bioorthogonal chemical reporter must be incorporated into a biomolecule. This occurs via metabolism. The chemical reporter is linked to a substrate, which a cell can metabolize.

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Bioorthogonal chemical reporter

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In chemical biology, bioorthogonal chemical reporter is a non-native chemical functionality that is introduced into the naturally occurring biomolecules...

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Bioorthogonal chemistry

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The term bioorthogonal chemistry refers to any chemical reaction that can occur inside of living systems without interfering with native biochemical processes...

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Thymidine kinase

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Pernot L, Schreier VN, Scapozza L, Luedtke NW (June 2015). "A Bioorthogonal Chemical Reporter of Viral Infection". Angewandte Chemie. 54 (27): 7911–4. doi:10...

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the term "bioorthogonal chemistry" for chemical reactions compatible with living systems. Her recent efforts include synthesis of chemical tools to study...

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a nitrogen insertion reaction: Some azides are valuable as bioorthogonal chemical reporters, molecules that can be "clicked" to see the metabolic path...

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of a living cell. Thus, chemists have recently developed a panel of bioorthogonal chemistry that proceed chemospecifically, despite the milieu of distracting...

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is their restricted bioorthogonality in certain cellular environments. Limitations of aldehydes and ketones as chemical reporters include: Competition...

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S2CID 2651429. Lang K, Chin JW (January 2014). "Bioorthogonal reactions for labeling proteins". ACS Chemical Biology. 9 (1): 16–20. doi:10.1021/cb4009292...

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order structure of DNA, non-sequence specific DNA binding proteins and chemical modification of DNA. In general epigenetic effects alter the accessibility...

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