Analytical method to determine potency and effect of a substance
Bioassay setup
A biological test system (here: Daphnia magna) is exposed to various experimental conditions (here: several microplastics preparations), to which it reacts.
Some indicator of these reactions (e.g. a color change) is assessed, typically in a highly automated fashion through microplates like this.
A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants (in vivo), or on living cells or tissues (in vitro).[1][2] A bioassay can be either quantal or quantitative, direct or indirect.[3] If the measured response is binary, the assay is quantal; if not, it is quantitative.[3]
A bioassay may be used to detect biological hazards or to give an assessment of the quality of a mixture.[4] A bioassay is often used to monitor water quality as well as wastewater discharges and its impact on the surroundings.[5] It is also used to assess the environmental impact and safety of new technologies and facilities.[citation needed]
Bioassays are essential in pharmaceutical, medical and agricultural sciences for development and launching of new drugs, vitamins, etc.
^Hoskins, W. M.; Craig, R. (1962-01-01). "Uses of Bioassay in Entomology". Annual Review of Entomology. 7 (1): 437–464. doi:10.1146/annurev.en.07.010162.002253. ISSN 0066-4170. PMID 14449182.
^"Guidance for Industry: Potency Tests for Cellular and Gene Therapy Products". Washington, D.C.: U.S. Food and Drug Administration. January 2011. p. 7.
^ abLaska, E M; Meisner, M J (1987-04-01). "Statistical Methods and Applications of Bioassay". Annual Review of Pharmacology and Toxicology. 27 (1): 385–397. doi:10.1146/annurev.pa.27.040187.002125. ISSN 0362-1642. PMID 3579242.
^Prinsloo, Gerhard; Papadi, Georgia; Hiben, Mebrahtom G.; Haan, Laura de; Louisse, Jochem; Beekmann, Karsten; Vervoort, Jacques; Rietjens, Ivonne M.C.M. (2017). "In vitro bioassays to evaluate beneficial and adverse health effects of botanicals: promises and pitfalls". Drug Discovery Today. 22 (8): 1187–1200. doi:10.1016/j.drudis.2017.05.002. PMID 28533190.
^"Permit Limits-Whole Effluent Toxicity (WET)". National Pollutant Discharge Elimination System (NPDES). Washington, D.C.: U.S. Environmental Protection Agency (EPA). 2021-10-11.
Bioassay setup A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants (in vivo)...
step-by-step separation of extracted components based on differences in their bioassay-guided fractionation physicochemical properties, and assessing the biological...
titration. On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or...
for NPDES permits, as well as Superfund cleanup levels. Industrial: This bioassay is used in the assessment of the toxicity of complex industrial effluent...
Y. & Bryant S H. (2014). The NCBI Handbook, 2nd edition, NCBI PubChem BioAssay Database Wikidata has the property: NCBI taxonomy ID (P685) (see uses)...
BioAssay Results". The PubChem Project. USA: National Center for Biotechnology Information. Retrieved 28 January 2016. "all[filt] - PubChem BioAssay Results"...
Hossain, Amjad; Hossain, Shaikat; Phelps, John (2014). "Human Sperm Bioassay". Medical and Surgical Management of Male Infertility. doi:10.5005/jp/books/11840_9...
environmental fate and transport, exposure, usage, in vivo toxicity, and in vitro bioassay. EPA and other scientists use the data and models contained within the...
of three phenylurea herbicides using Lemna minor and Vibrio fischeri bioassays. Chemosphere vol. 119, January 2015, p. 569-574. Gatidou et al., 2015...
of Synthetic Cannabinoids and Their Metabolites with a Newly Developed Bioassay". Analytical Chemistry. 88 (23): 11476–11485. doi:10.1021/acs.analchem...
"Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC". In Barreiro C, Barredo JL (eds.). Antimicrobial Therapies. Methods...
exposed to the intake of radioactive particulates can be estimated using bioassay data such as lung and body counter measurements, urine or faecal radioisotope...
refinery workers. This is supported by the positive results of the NTP bioassays with Ni sub-sulfide and Ni oxide in rats and mice. The human and animal...
que qin jiao) is a plant species in the genus Gentiana found in Asia. Bioassay-directed fractionation techniques led to isolation of isoorientin as the...
about 5-fold stronger than that of testosterone (EC50 = 0.66 nM). In bioassays, DHT has been found to be 2.5- to 10-fold more potent than testosterone...
Antonio Pereira de (2017-12-07). "Evaluation of eugenol toxicity in bioassays with test-organisms". Ciência Rural. 47 (12). doi:10.1590/0103-8478cr20170194...
for studying drugs that modify neurotransmission. It has been used: as a bioassay for the discovery of enkephalins, the endogenous opiates. to demonstrate...
petition by McNeil Nutritionals, the FDA stated that "in the 2-year rodent bioassays... there was no evidence of carcinogenic activity for either sucralose...
co;2. PMID 18459401. Willem G. van Herk; Robert S. Vernon (2007). "Soil bioassay for studying behavioral responses of wireworms (Coleoptera: Elateridae)...
equivalent (BEQ) is a unit of measure in the field of bioassays. It is used for example in the bioassay CALUX for testing dioxins and dioxin-like compounds...
phosphorus, chlorine. Biological characteristics are determined with bioassays and aquatic toxicology tests. Sewage can be monitored for both disease-causing...
various techniques such as chromatography or by a simple agar diffusion bioassay. Nisin is used in processed cheese, meats, beverages, etc. during production...
analytical methods can be used to identify and quantify YTXs. The mouse bioassay (MBA) procedure developed by Yasumoto et al. is the official reference...
potent anti-inflammatory compound in a variety of in vitro and in vivo bioassay models, oral quercetin in human subjects has not exhibited the desired...
Wissenschaftliche Zoologie. 135: 323–356. Nissani M (May 1975). "A new behavioral bioassay for an analysis of sexual attraction and pheromones in insects". The Journal...
animals. As early as 2001 an antemortem test was deemed urgent. Running a bioassay, taking fluids from animals in the deer family suspected of infection and...
role January 1, 1965 U.S. Testing Environmental & bioassay testing Assumed GEs environmental and bioassay testing role January 4, 1965 Battelle Memorial...
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