An antibody elution removes bound antibody from the surface of a red blood cell to aid in the antibody identification process.
An antibody elution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the blood group system antigen the antibody targets.[1] An antibody elution is deemed necessary when antibodies of the immunoglobulin class G (IgG) are found sensitized (bound) to peripheral red cells collected from a blood product transfusion recipient.[2] IgG antibodies are detected using an assay known as the direct antiglobulin test.[3]
Antibody elutions are specialized tests used in clinical blood banks. Examples of routine tests include ABO/Rh, antibody screen, antibody identification, and antiglobulin testing. Examples of other specialized tests used in blood banking include: treatment with thiol reagent, monocyte monolayer assay, enzyme treatment, and adsorptions.[2]
This procedure aids in the investigation of antibodies that are difficult to identify, distinguishing transfusion reactions, hemolytic disease of the fetus and newborn, and warm autoantibody workups.[4]
An antibodyelution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the blood...
elute in the same order and at the same elution volume. Antibodyelution is the process of removing antibodies that are attached to their targets, such...
are washed away. An elution with a low pH buffer or a more gentle, high salt elution buffer is then used to recover purified antibody from the support....
red blood cell alloantibodies in recipient serum. Antibodyelution is the process of removing antibodies from the surface of red blood cells. Techniques...
desorbed by an elution buffer. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. After elution, the adsorbent...
matrix. This allows any antibodies that recognize the antigen to be captured on the solid support. Elution of the antibodies of interest is most often...
by using 'bacterial packaging cell line' technology. Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening...
fragmentation buffer, immunoprecipitation with purified anti-m6A antibody, elution and collection of antibody-tagged RNA molecules. The immunoprecipitation procedure...
therapy stent combines the anti-CD34 antibody coating of the Genous Stent with antiproliferative sirolimus elution. The sirolimus drug reduces the risk...
Jka and/or Jkb can be detected on In(Jk) red blood cells in adsorption/elution tests. Anti-Jka and -Jkb are not common. They are usually warm-reacting...
prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences...
affecting reproducibility in proteomics experiments is the simultaneous elution of many more peptides than mass spectrometers can measure. This causes...
molecules, thus separate them. This type of elution is called gradient elution. On the other hand, step elution can be used, in which the concentration of...
bound phages with strong affinity are kept. The final step involves the elution step where the bound phages are eluted through changing of pH or other...
combines Genous with an antiproliferative, biodegradable sirolimus drug elution. The Combo Stent was shown to be as effective as a paclitaxel-eluting stent...
acetonitrile), can be used to increase the mobile phase polarity to affect elution These salts are not volatile, so this technique is less useful with a mass...
structure metadatabase GlycoBase A web HPLC/UPLC resource that contains elution positions expressed as glucose unit values. ProGlycAn A short introduction...
identification of the antibody. Among these techniques are elutions, adsorptions, and enzyme treatment. Some patients produce antibodies to high frequency...
selective Protein a resin allows for mild sodium chloride-mediated elution of antibodies". Journal of Chromatography A. 1637: 461843. doi:10.1016/j.chroma...
chromatography due to their thermolability. In place of solvent gradient elution, thermoresponsive polymers allow the use of temperature gradients under...
immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody". J Biol Chem. 265: 7069–7077...
repulsion with negatively charged functional groups on biomolecules. For the elution of molecules buffer with high concentration of phosphate and sodium chloride...
the fluidized bed is washed to flush out any remaining particulates. Elution of the adsorbed proteins was commonly performed with the eluent flow in...
guanidine HCl (6–8 M), giving it a lower dissociation KD ≈ 10−7M. This allows elution from the avidin matrix to occur under milder, non-denaturing conditions...
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