The Affimer protein scaffold – showing the two loops where random peptides can be inserted to create a protein binding surface
Affimer molecules[1] are small proteins that bind to target proteins with affinity in the nanomolar range.[2][3] These engineered non-antibody binding proteins are designed to mimic the molecular recognition characteristics of monoclonal antibodies in different applications.[2][4] These affinity reagents have been optimized to increase their stability,[5] make them tolerant to a range of temperatures and pH,[6] reduce their size, and to increase their expression in E.coli and mammalian cells.[2]
^Proprietary name, owned by Avacta
^ abcTiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, et al. (June 2017). "Affimer proteins are versatile and renewable affinity reagents". eLife. 6. doi:10.7554/elife.24903. PMC 5487212. PMID 28654419.
^Klont F, Hadderingh M, Horvatovich P, Ten Hacken NH, Bischoff R (August 2018). "Affimers as an Alternative to Antibodies in an Affinity LC-MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum". Journal of Proteome Research. 17 (8): 2892–2899. doi:10.1021/acs.jproteome.8b00414. PMC 6079930. PMID 30005571.
^"Antibody Alternatives". The Scientist Magazine®. Retrieved 2018-10-18.
^Sharma R, Deacon SE, Nowak D, George SE, Szymonik MP, Tang AA, Tomlinson DC, Davies AG, McPherson MJ, Wälti C (June 2016). "Label-free electrochemical impedance biosensor to detect human interleukin-8 in serum with sub-pg/ml sensitivity". Biosensors & Bioelectronics. 80: 607–613. doi:10.1016/j.bios.2016.02.028. PMC 4785862. PMID 26897263.
Affimer molecules are small proteins that bind to target proteins with affinity in the nanomolar range. These engineered non-antibody binding proteins...
recognition applications. Affimer technology has been commercialised and developed by Avacta Life Sciences, who are developing Affimer binders as reagents for...
website by PBS/NOVA 1999 Mallory & Irvine Research Expedition website by AFFIMER Mallory and Irvine Research expedition 2001 Mallory and Irvine Research...
detecting the same class of immunoglobulins with the same specificity. Affimer Anti-mitochondrial antibodies Anti-nuclear antibodies Antibody mimetic...
development include avelumab and durvalumab, in addition to an inhibitory affimer. Other modes of enhancing [adoptive] immuno-therapy include targeting so-called...
A, Song Q, Ko Ferrigno P, Bueno PR, Davis JJ (Aug 7, 2012). "Sensitive Affimer and antibody based impedimetric label-free assays for C-reactive protein"...
was acylated with fatty acyl residues of 6-18 carbon atoms.[relevant?] Affimer, a type of engineered protein that is based on the cystatin scaffold Salát...
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