TRAMP complex (Trf4/Air2/Mtr4p Polyadenylation complex) is a multiprotein, heterotrimeric complex having distributive polyadenylation activity and identifies wide varieties of RNAs produced by polymerases. It was originally discovered in Saccharomycescerevisiae by LaCava et al., Vanacova et al. and Wyers et al. in 2005.[1]
The TRAMP complex consists of RNA helicase (Mtr4/Dob1), a poly(A) polymerase (either Trf4 or Trf5) and a zinc knuckle protein (either Air1 or Air2).The interaction between the exosomes (Rrp6p/Rrp47p) and Mtr4p of TRAMP complex helps in RNA surveillance and degradation of abnormal RNAs
It interacts with the exosome complex in the nucleus of eukaryotic cells and is involved in the 3' end processing and degradation of ribosomal RNA and snoRNAs.[1][2] The TRAMP complex trims the poly(A) tails of RNAs destined for Rrp6 and the core exosome down to 4-5 adenosines assisting in transcript recognition and exosome complex activation.[1][3] The substrate specificity of exosomes is improved in the presence of TRAMP complex as it acts as a crucial cofactor and helps in maintaining various activities.[4]
In this way, TRAMP plays a critical role in ridding the cell of noncoding transcripts generated through pervasive RNA polymerase II transcription, as well as functioning in the biogenesis and turnover of functional coding and noncoding RNAs.[5]
TRAMP complex also affects various other RNA processes either directly or indirectly. It is involved in RNA export, Splicing, hetero-chromatic gene silencing and helps in maintaining stability of genome.[6]
^ abcJia, Huijue; Wang, Xuying; Liu, Fei; Guenther, Ulf-Peter; Srinivasan, Sukanya; Anderson, James T.; Jankowsky, Eckhard (2011-06-10). "The RNA helicase Mtr4p modulates polyadenylation in the TRAMP complex". Cell. 145 (6): 890–901. doi:10.1016/j.cell.2011.05.010. ISSN 1097-4172. PMC 3115544. PMID 21663793.
^Callahan, Kevin P.; Butler, J. Scott (2010-02-05). "TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6". The Journal of Biological Chemistry. 285 (6): 3540–3547. doi:10.1074/jbc.M109.058396. ISSN 1083-351X. PMC 2823493. PMID 19955569.
^Schmidt, Karyn; Butler, J. Scott (March 2013). "Nuclear RNA surveillance: role of TRAMP in controlling exosome specificity". Wiley Interdisciplinary Reviews: RNA. 4 (2): 217–231. doi:10.1002/wrna.1155. ISSN 1757-7012. PMC 3578152. PMID 23417976.
^Ciais, Delphine; Bohnsack, Markus T.; Tollervey, David (May 2008). "The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3' processing pathway". Nucleic Acids Research. 36 (9): 3075–3084. doi:10.1093/nar/gkn160. ISSN 1362-4962. PMC 2396412. PMID 18400782.
^Genome integrity : facets and perspectives. Lankenau, Dirk-Henner. Berlin: Springer. 2007. ISBN 9783540375319. OCLC 164366985.{{cite book}}: CS1 maint: others (link)
TRAMPcomplex (Trf4/Air2/Mtr4p Polyadenylation complex) is a multiprotein, heterotrimeric complex having distributive polyadenylation activity and identifies...
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co-factor is the TRAMPcomplex, which is responsible for poly-adenylating transcripts and marking them for degradation. The TRAMPcomplex was discovered...
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(November 15, 2023). "Did Ice Spice Just Tease Her Next Project With a Tramp Stamp?". Teen Vogue. Retrieved January 27, 2024. Rettig, James (January...
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Time VII: The Stone of Cold Fire Sierra Direct-to-video 2001 Lady and the Tramp II: Scamp's Adventure Tony Shrek Captain of Guards Atlantis: The Lost Empire...
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