The SpyTag/SpyCatcher system is a technology for irreversible conjugation of recombinant proteins. The peptide SpyTag (13 amino acids) spontaneously reacts with the protein SpyCatcher (12.3 kDa) to form an intermolecular isopeptide bond between the pair.[1] DNA sequence encoding either SpyTag or SpyCatcher can be recombinantly introduced into the DNA sequence encoding a protein of interest, forming a fusion protein. These fusion proteins can be covalently linked when mixed in a reaction through the SpyTag/SpyCatcher system.
Using the Tag/Catcher pair, bioconjugation can be achieved between two recombinant proteins that would otherwise be restrictive or impossible with traditional direct genetic fusion between the two proteins. For example, issues regarding protein folding, suboptimal expression host, and specialized post-translational modifications can be alleviated by separating the production of the proteins with the modularity of the Tag/Catcher system.[2]
^Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M (March 2012). "Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin". Proceedings of the National Academy of Sciences of the United States of America. 109 (12): E690-7. doi:10.1073/pnas.1115485109. PMC 3311370. PMID 22366317.
^Brune KD, Howarth M (26 June 2018). "New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue". Frontiers in Immunology. 9: 1432. doi:10.3389/fimmu.2018.01432. PMC 6028521. PMID 29997617.
The SpyTag/SpyCatcher system is a technology for irreversible conjugation of recombinant proteins. The peptide SpyTag (13 amino acids) spontaneously reacts...
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utilizing the chemistry of isopeptide bond conjugation using the SpyTag/SpyCatcher technology. This involves having a streptavidin tetramer with three...
(TDKDMTITFTNKKDAE) SpyTag, a peptide which binds covalently to SpyCatcher protein (AHIVMVDAYKPTK) SnoopTag, a peptide which binds covalently to SnoopCatcher protein...
passing nasty notes about her in class. In return, Harriet regularly spies on the SpyCatcher Club through a back fence and concocts vengeful ways to punish...
distribution of Spycatcher, it was finally published in 1987 and eventually sold over two million copies around the world. Wright alleges in Spycatcher that Gouzenko...
knew. Peter Wright, one of Blunt's interrogators, describes in his book Spycatcher how Blunt was evasive and only made admissions grudgingly, when confronted...
Pinto published two books, Spy-catcher and Friend or Foe? These formed the basis of the 1959-1961 BBC television series Spycatcher, and also an earlier BBC...
this, other Tag/Catcher systems were developed such as SnoopTag/SnoopCatcher and SdyTag/SdyCatcher that complement SpyTag/SpyCatcher. Organic Chemistry...
(1987). Spycatcher: The Candid Autobiography of a Senior Intelligence Officer. Stoddart Publishing. p. 128. Ferguson, Donna (23 May 2021). "Spy who got...
insufficient evidence to support the allegations. Peter Wright in his memoirs, Spycatcher, discusses Trend and his report. Trend married Patricia Charlotte Shaw...
production without having seen a script. He made the pilot episode, "SpyCatcher", which was shot in November 1970. The pilot impressed Paramount, who...
paralysed when such agents defected to the Soviet Union; in his book, Spycatcher, he suggests that his hypothesis had to be true, and that the Soviets...
in German-occupied France during World War II. Described as a "super spy-catcher," Bleicher infiltrated resistance networks in France and was responsible...
one point close to Peter Wright. Pincher became ensnared in 1986 in the Spycatcher affair, when Wright tried to publish his own book in Australia, in apparent...
MI5 from 1992 to 1996); and Matthew Dunn's Spycatcher (2011) and sequels. Much spy fiction was adapted as spy films in the 1960s, ranging from the fantastical...
many vocal supporters of Ellis. Watt rubbished Wright's 1987 bestseller Spycatcher and the case against Ellis in a 1988 essay for The Political Quarterly:...
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Sullivan (from the second season). Spy Trap was created by Robert Barr, who also wrote the earlier BBC TV series Spycatcher, and was notable for its complex...
leak was published on the website Guido Fawkes. According to the book Spycatcher by Peter Wright (published in 1987), the technique is standard practice...
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