Photoacoustic microscopy is an imaging method based on the photoacoustic effect and is a subset of photoacoustic tomography. Photoacoustic microscopy takes advantage of the local temperature rise that occurs as a result of light absorption in tissue. Using a nanosecond pulsed laser beam, tissues undergo thermoelastic expansion, resulting in the release of a wide-band acoustic wave that can be detected using a high-frequency ultrasound transducer.[1] Since ultrasonic scattering in tissue is weaker than optical scattering, photoacoustic microscopy is capable of achieving high-resolution images at greater depths than conventional microscopy methods. Furthermore, photoacoustic microscopy is especially useful in the field of biomedical imaging due to its scalability. By adjusting the optical and acoustic foci, lateral resolution may be optimized for the desired imaging depth.[2]
^H.F. Zhang; K. Maslov; G. Stoica; L.V. Wang (2006). "Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging" (PDF). Nature Biotechnology. 24 (7): 848–851. doi:10.1038/nbt1220. PMID 16823374. S2CID 912509.
^L.V. Wang; J. Yao (2013). "Photoacoustic Microscopy". Laser Photonics Rev. 7 (5): 10. Bibcode:2013LPRv....7..758Y. doi:10.1002/lpor.201200060. PMC 3887369. PMID 24416085.
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Quaroni and University of Saskatchewan professor Alan Casson used infrared microscopy to identify biomarkers inside individual cells from tissue associated...