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The partial specific volume express the variation of the extensive volume of a mixture in respect to composition of the masses. It is the partial derivative of volume with respect to the mass of the component of interest.
where is the partial specific volume of a component defined as:
The PSV is usually measured in milliLiters (mL) per gram (g), proteins > 30 kDa can be assumed to have a partial specific volume of 0.708 mL/g.[1] Experimental determination is possible by measuring the natural frequency of a U-shaped tube filled successively with air, buffer and protein solution.[2]
^Buxbaum, Engelbert (2011). "25: Centrifugation". Biophysical Chemistry of Proteins. New York, Dordrecht, Heidelberg, London: Springer. pp. 237–249. doi:10.1007/978-1-4419-7251-4_25. ISBN 978-1-4419-7250-7.
^Kratky, O.; Leopold, H.; Stabinger, H. (1973). "5: The determination of the partial specific volume of proteins by the mechanical oscillator technique". In Hirs, C.H.W.; Timasheff, S.N. (eds.). Meth. Enzymol. Vol. 27: Enzyme Structure Part D. pp. 98–110. ISBN 978-0-12-181890-6.
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