MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome,[1] which is used for biochemical purification of RNA-protein complexes and partnered to GFP for detection of RNA in living cells.[2] More recently, the technique has been used to monitor the appearance of RNA in living cells, at the site of transcription, or simply by observing the changes in RNA number in the cytoplasm.[3][4] This has revealed that transcription of both prokaryotic and eukaryotic genes occurs in a discontinuous fashion with bursts of transcription separated by irregular intervals.
^Johansson HE, Liljas L, Uhlenbeck OC (1997). "RNA recognition by the MS2 phage coat protein". Seminars in Virology. 8 (3): 176–185. doi:10.1006/smvy.1997.0120.
^Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM (October 1998). "Localization of ASH1 mRNA particles in living yeast". Molecular Cell. 2 (4): 437–45. doi:10.1016/s1097-2765(00)80143-4. PMID 9809065.
^Golding I, Paulsson J, Zawilski SM, Cox EC (December 2005). "Real-time kinetics of gene activity in individual bacteria". Cell. 123 (6): 1025–36. doi:10.1016/j.cell.2005.09.031. PMID 16360033. S2CID 10319035.
^Chubb JR, Trcek T, Shenoy SM, Singer RH (May 2006). "Transcriptional pulsing of a developmental gene". Current Biology. 16 (10): 1018–25. doi:10.1016/j.cub.2006.03.092. PMC 4764056. PMID 16713960.
MS2tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome, which...
Bacteriophage MS2 (Emesvirus zinderi), commonly called MS2, is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia...
to the MS2 protein without disrupting the dCas9-sgRNA complex. Thus, the MS2 protein is engineered to include p65 and HSF1 proteins. The MS2-p65-HSF1...
This phenomenon came to light with the advent of technologies, such as MS2tagging and single molecule RNA fluorescence in situ hybridisation, to detect...
tags appear as a single composite peak at the same m/z value in an MS1 scan with identical liquid chromatography (LC) retention times. During the MS2...
Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass...
technique, the RNA of interest is tagged to an adenosine rich reporter motif that consists of multiple MS2 binding sites. These MS2 binding sites recruit a complex...
sensor, and many more. See the Megamanual for download and documentation MS2/Extra code This is an extension of the base code, adding many of the much...
protein mass spectrometry, tandem mass spectrometry (also known as MS/MS or MS2) experiments are used for protein/peptide identification. Peptide identification...
sequence of the first complete gene and the complete genome of Bacteriophage MS2, identified and published by Walter Fiers and his coworkers at the University...
these genera (such as the Fiersviridae MS2 and the Quebevirus Qβ), and is phylogenetically classified between MS2 and Qβ. The operator region of displays...
the 1970s, new techniques for sequencing DNA were applied to bacteriophage MS2 and øX174, and the extended nucleotide sequences were then parsed with informational...
complete nucleotide sequence of an RNA virus genome, that of bacteriophage MS2. In 1977, introns and RNA splicing were discovered in both mammalian viruses...
particles have the smallest genomes in nature. For instance, bacteriophage MS2 consists of only 3569 nucleotides (single-stranded RNA) and encodes just...
were the first to determine the sequence of a gene: that of bacteriophage MS2 coat protein. The subsequent development of chain-termination DNA sequencing...
5386 base pairs in length), shortly after the RNA genome of bacteriophage MS2 (in 1976). T4 phage Animal viruses: SV40 Human alphaherpesvirus (Herpes simplex...
aspect is effectively announced. Semi-automatic signals on a station are tagged with consecutive letters of Latin alphabet, or with a letter followed by...
kinetic energy spectrometry MS – Mass spectrometer MS – Mass spectrometry MS2 – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry MS/MS...
of use compared to other word processing options, though the high price tag was criticized, especially in comparison to the IBM 5150 PC and other compatibles...
Replication of viral RNA, 8. Studies on the enzymatic mechanism of replication of MS2 RNA. Proc Natl Acad Sci U S A. 1965;54:982-7. Van Bruggen EF, Borst P, Ruttenberg...
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