mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish.[1] Fluorescent proteins are used to tag components in cells so that they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540 and 590 nm and emits light in the range of 550-650 nm.[2] mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. Genome editing has been improved greatly through the precise insertion of these fluorescent protein tags into the genetic material of many diverse organisms. Most comparisons between the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that affect protein performance in cells or organisms.[3] It is hard to perfectly simulate cellular environments in vitro, and the difference in environment could have an effect on the brightness and photostability.
mRFPs—like mCherry—are useful because they have a lower molecular weight and fold faster than tetramers, which results in reduced disturbance of the target system.
^Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004-11-21). "Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein". Nature Biotechnology. 22 (12): 1567–1572. doi:10.1038/nbt1037. ISSN 1087-0156. PMID 15558047. S2CID 205272166.
^Shu, Xiaokun; Shaner, Nathan C.; Yarbrough, Corinne A.; Tsien, Roger Y.; Remington, S. James (August 2006). "Novel Chromophores and Buried Charges Control Color in mFruits". Biochemistry. 45 (32): 9639–9647. doi:10.1021/bi060773l. ISSN 0006-2960. PMID 16893165.
^Heppert, Jennifer K.; Dickinson, Daniel J.; Pani, Ariel M.; Higgins, Christopher D.; Steward, Annette; Ahringer, Julie; Kuhn, Jeffrey R.; Goldstein, Bob (2016-11-07). "Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system". Molecular Biology of the Cell. 27 (22): 3385–3394. doi:10.1091/mbc.e16-01-0063. ISSN 1059-1524. PMC 5221575. PMID 27385332.
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones...
nucleoid B. Cross-sectioning of the E. coli nucleoid visualized by HU-mCherry. Fluorescence intensity is taken as a proxy for DNA density and is represented...
half-life of 24 hour. smURFP is extremely photostable and out performs mCherry and tdTomato in living cells. Single-molecule smURFPs emit twice as many...
Drosophila larva whose tracheal cells are marked with membrane-tethered mCherry fluorescent protein. Some of the cytonemes that extend from the tracheal...
construct (green), while the chromosomal terminus is labeled with MatP-mCherry (red). A phase contrast image (gray) is overlaid to visualize the cell...
cellular proteins are made with conjugated fluorescent tags such as GFP, mCherry, and Luciferase that can be used to detect and quantify those components...
evolution, he has created fluorescent proteins, including mNeonGreen, mCherry, and dTomato. Shaner discovers new, naturally occurring bioluminescent...
original on 9 June 2010. Retrieved 4 March 2010. Fan, J. Y. et al. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing...
(green; GFP-expressing cells) and normal human astrocytes (NHA) (red; mCherry-labelled) cultured separately and then magnetically guided together (left...
fluorescent reporter proteins such as Green Fluorescent Protein (GFP) and mCherry (red) or bioluminescent reporters such as Luciferase. These transgenic...
receptor exclusively activated by a designer drug (DREADD; AAV8-hSyn-hM4Di-mCherry). By contrast, two non-episodic memory assessments are unaffected by CNO...
such that the resultant protein is tagged by the red fluorescent protein "mcherry"; thus, a red fluorescence by the microbe will signify the successful creation...
nanoCLAMPs have been produced that target green fluorescent protein (GFP), mCherry, SUMO (SMT3), NusA, avidin, NeutrAvidin, maltose-binding protein (MBP)...
eGFP and mCherry fluorescent proteins have been imaged in model organisms such as Drosophila melanogaster pupae and adult zebrafish, and mCherry has been...
PMID 11792318. S2CID 14030684. Renn J, Winkler C (January 2009). "Osterix-mCherry transgenic medaka for in vivo imaging of bone formation". Developmental...
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