In molecular biology, linker DNA is double-stranded DNA (38-53 base pairs long) in between two nucleosome cores that, in association with histone H1, holds the cores together. Linker DNA is seen as the string in the "beads and string model", which is made by using an ionic solution on the chromatin. Linker DNA connects to histone H1 and histone H1 sits on the nucleosome core. Nucleosome is technically the consolidation of a nucleosome core and one adjacent linker DNA; however, the term nucleosome is used freely for solely the core. Linker DNA may be degraded by endonucleases.[1]
The linkers are short double stranded DNA segments which are formed of oligonucleotides. These contain target sites for the action of one or more restriction enzymes. The linkers can be synthesized chemically and can be ligated to the blunt end of foreign DNA or vector DNA. These are then treated with restriction endonuclease enzyme to produce cohesive ends of DNA fragments. The commonly used linkers are EcoRI-linkers and sal-I linkers.
^Molecular Biology of The Cell, Fifth Edition, Alberts et al., Garland Science, 2008
In molecular biology, linkerDNA is double-stranded DNA (38-53 base pairs long) in between two nucleosome cores that, in association with histone H1,...
the DNA of histone H1 binding to the nucleosome. Histone H1, and its other variants, are referred to as linker histones. Protruding from the linker histone...
Look up linker in Wiktionary, the free dictionary. Linker or linkers may refer to: Linker (computing), a computer program that takes one or more object...
of the 2 DNA, homogenous bonds are forming. The basic repeat element of chromatin is the nucleosome, interconnected by sections of linkerDNA, a far shorter...
linkerDNA is shortened, or whether it merely promotes a change in the angle of adjacent nucleosomes, without affecting linker length However, linker...
exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble "beads on a string of DNA" under an electron...
DNA repair pathway, including DNA-PKcs, Ku70/Ku80 and DNA ligase IV assembled with topo IIβ and PARP-1. This assemblage was all present at the linker...
biology, the linker histone H1 is a protein family forming a critical component of eukaryotic chromatin. H1 histones bind to the linkerDNA exiting from...
Deoxyribonucleic acid (/diːˈɒksɪˌraɪboʊnjuːˌkliːɪk, -ˌkleɪ-/ ; DNA) is a polymer composed of two polynucleotide chains that coil around each other to...
because the DNA is normally tightly wrapped around histones, the core proteins of the nucleosomes. The linker sites are the only parts of the DNA strand that...
DNA supercoiling refers to the amount of twist in a particular DNA strand, which determines the amount of strain on it. A given strand may be "positively...
molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule. DNA replication occurs...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
domain that are connected via the linker region, which binds the accessory subunit. The accessory subunit binds DNA and is required for processivity of...
strand are linked together via the histone, H1, and a short space of open linkerDNA, ranging from around 0-80 base pairs. The key distinction between the...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
DNA (aDNA) is DNA isolated from ancient specimens. Due to degradation processes (including cross-linking, deamination and fragmentation) ancient DNA is...
biotinylated linker is designed to form a hairpin structure that specifically labels DSBs and not single-strand DNA breaks. The linker has a blunt, ligatable...
the untrained leg, had significant DNA methylation changes at 4,919 sites across the genome. The sites of altered DNA methylation were predominantly in...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method". Environ. Microbiol...
(linker histones), H2, H3, and H4 (core histones). The nucleosome core is formed of two H2A-H2B dimers and a H3-H4 tetramer. The tight wrapping of DNA...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linkerDNA between nucleosomes and...
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