Chemical used for the detection and quantification of bacterial endotoxins
Atlantic horseshoe crab Limulus polyphemus
Limulus amebocyte lysate (LAL) is an aqueous extract of motile blood cells (amebocytes) from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts with bacterial endotoxins such as lipopolysaccharides (LPS), which are components of the bacterial capsule, the outermost membrane of cell envelope of gram-negative bacteria. This reaction is the basis of the LAL test, which is widely used for the detection and quantification of bacterial endotoxins.
In Asia, a similar Tachypleus amebocyte lysate (TAL) test based on the local horseshoe crabs Tachypleus gigas or Tachypleus tridentatus is occasionally used instead.[1] The recombinant factor C (rFC) assay is a replacement of LAL and TAL based on a similar reaction.[2]
^Sheng J, Zhou J, Peng Y, Zhu Z, Chen L (1 January 2006). "Tachypleus Amebocyte Lysate Test Using in Transfusion Reaction". Chinese Journal of Nosocomiology (in Chinese).
^"Endotoxin Testing Manufacturers and Conservation". www.horseshoecrab.org. Retrieved 10 March 2020.
and 17 Related for: Limulus amebocyte lysate information
Limulusamebocytelysate (LAL) is an aqueous extract of motile blood cells (amebocytes) from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts...
caught for use as fishing bait, in biomedicine (especially for Limulusamebocytelysate) and science. They play a major role in the local ecosystems, with...
prolate-to-fusiform shape. Limulusamebocytelysate, an aqueous extract of amebocytes from the Atlantic horseshoe crab (Limulus polyphemus), is commonly...
used as fishing bait, in fertilizer and in science (especially Limulusamebocytelysate, which is used for the detection and quantification of bacterial...
than the mammalian fibrin clot. Limulusamebocytelysate is found only in horseshoe crabs, specifically the Limulus polyphemus species. In the presence...
2 more Sushi domains a trypsin domain This enzyme is useful in Limulusamebocytelysate as the endotoxin-detecting element. It can be produced recombinantly...
additive. The blood of horseshoe crabs contains a clotting agent, LimulusAmebocyteLysate, which is now used to test that antibiotics and kidney machines...
detecting presence of endotoxin is the LimulusAmebocyteLysate (LAL) assay, utilizing blood from the Horseshoe crab (Limulus polyphemus). Very low levels of...
purposes as emu oil.) Horn, including antlers etc. Ivory Lanolin Limulusamebocytelysate (a chemical in horseshoe crab blood used to detect bacterial endotoxin)...
meat a bad taste. Horseshoe crab blood contains a clotting agent, limulusamebocytelysate, which is used to test antibiotics and kidney machines to ensure...
level. Currently, one common method for endotoxin detection is the LimulusAmebocyteLysate (LAL) test. This test is based on Dr. Frederik Bang's observation...
(1916–1981) was an American medical researcher who developed the Limulusamebocytelysate (LAL) test for bacterial endotoxins. He was influential in applying...
domain of life. U.S. Food and Drug Administration approves LAL (Limulusamebocytelysate) for testing drugs, products and devices that come in contact with...
horseshoe crab blood production. Horseshoe crab blood contains limulusamebocytelysate (LAL), which is the gold standard in validating medical equipment...
epidemiologist Betsy Bang – biologist Fred Bang – developed the Limulusamebocytelysate (LAL) test for bacterial endotoxins Florence Bascom – geologist...
non viable biological material so can give a "false positive". LimulusAmebocyteLysate assay - detects lipopolysaccharides (LPS). This compound is only...
and glucan are detected with the Limulusamebocytelysate (LAL) enzyme cascade, derived from the horseshoe crab Limulus polyphemus. Lipoteichoic acid is...
Global Information