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HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from certain region as demonstrated below.[1] It has the ability to produce cohesive ends, which are rather useful in constructing plasmids.
Recognition sequence
Cut results
5' CCGG
3' GGCC
5' C CGG
3' GGC C
HpaII will not cut sites that have been methylated by SssI methyltransferase or HpaII methyltransferase. When the sites have been methylated by MspI methyltransferase, the enzyme will cut 300 times slower than unmethylated DNA and 50 times slower if the DNA is hemi-methylated.[2] This feature is exploited for determination of the clonal origin of a mammalian female tumor through HUMARA assay.
^InterPro: IPR019062
^McClelland, M., Nelson, M., Raschke, E. (1994). Nucleic Acids Research 22, No. 17, 3640-3659.
HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore...
the restriction nucleases MspI and HpaII, both of which recognize and cleave within the same sequence. However, HpaII requires that a C within that site...
isolating it from a bacterial strain. For example, the restriction enzymes HpaII and MspI are isoschizomers, as they both recognize the sequence 5'-CCGG-3'...
For the purpose of DNA replication, the HpaII tiny fragment Enrichment by Ligation-mediated PCR Assay (HELP Assay) is one of several techniques used for...
also has certain base orders that make it susceptible to be digested by HpaII (or HhaI) enzyme when it is not methylated. This detail gives the opportunity...
such as HpaII). It is also known that BstUI can cut at unconverted sites, leading to overestimation of methylation levels and so the use of HpaII is often...
MeCP2. He led the team which first identified CpG islands—originally named "HpaII tiny fragments"—in vertebrate genomes. These are short genomic regions with...
Tyetkheperre Setepenre Pasebakhaenniut Meryamun "who is simultaneously called the HPA (i.e., High Priest of Amun) and supreme military commander." This suggests...
formerly known as Karen State, is a state of Myanmar. The capital city is Hpa-An, also spelled Pa-An. The terrain of the state is mountainous with the...
question marks, boxes, or other symbols instead of Burmese script. Shin Hpa (Burmese: ရှင်ဘား, pronounced [ʃɪ̀ɴ pʰá]) was a queen consort of King Narathihapate...
Bird, and took part in the characterisation of what were then termed HTF (HpaII Tiny Fragments) islands in mammalian genomes. She and her PhD student Margaret...
cover different areas of the body. Hypothalamic–pituitary–adrenal axis (HPA) suppression Cushing's syndrome Diabetes mellitus Osteoporosis Topical steroid...
Cross in Silver on 2 October 1942 as Generalleutnant in the OKH/Chief of HPA Citations Patzwall & Scherzer 2001, p. 545. Bibliography Patzwall, Klaus...
Registration Form: Chicago Park Boulevard System Historic District" (PDF). gis.hpa.state.il.us. November 9, 2018. Archived from the original (PDF) on February...
Air PEARL LINE Pakistan PBY Pearl Air Services PEARL SERVICES Uganda HP HPA Pearl Airways PEARL AIRWAYS Haiti IATA code withdrawn PVU Peau Vavaʻu PEAU...
derived from pBluescript. pGreenII 0029: derived from pGreenII 0000, a nos-kan cassette has been inserted into the HpaI site of the Left Border, providing...
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