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Environmental scanning electron microscope information


Wool fibers imaged in an ESEM by the use of two symmetrical plastic scintillating backscattered electron detectors. Pseudocolor.
Fungal spores in lemon grass leaf, SE image, ElectroScan E3 ESEM.
Piece of a crystallized polystyrene latex, SE image with ElectroScan 2020 ESEM.

The environmental scanning electron microscope (ESEM) is a scanning electron microscope (SEM) that allows for the option of collecting electron micrographs of specimens that are wet, uncoated, or both by allowing for a gaseous environment in the specimen chamber. Although there were earlier successes at viewing wet specimens in internal chambers in modified SEMs, the ESEM with its specialized electron detectors (rather than the standard Everhart-Thornley detector) and its differential pumping systems, to allow for the transfer of the electron beam from the high vacuum in the gun area to the high pressure attainable in its specimen chamber, make it a complete and unique instrument designed for the purpose of imaging specimens in their natural state. The instrument was designed originally by Gerasimos Danilatos while working at the University of New South Wales.

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Environmental scanning electron microscope

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