Basic principle of tomography: superposition free tomographic cross sections S1 and S2 compared with the projected image P
Electron tomography (ET) is a tomography technique for obtaining detailed 3D structures[1] of sub-cellular, macro-molecular, or materials specimens. Electron tomography is an extension of traditional transmission electron microscopy and uses a transmission electron microscope to collect the data. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. This information is collected and used to assemble a three-dimensional image of the target. For biological applications, the typical resolution of ET systems[2] are in the 5–20 nm range, suitable for examining supra-molecular multi-protein structures, although not the secondary and tertiary structure of an individual protein or polypeptide.[3][4] Recently, atomic resolution in 3D electron tomography reconstructions has been demonstrated.[5][6]
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^R. A. Crowther; D. J. DeRosier; A. Klug (1970). "The Reconstruction of a Three-Dimensional Structure from Projections and its Application to Electron Microscopy". Proc. R. Soc. Lond. A. 317 (1530): 319–340. Bibcode:1970RSPSA.317..319C. doi:10.1098/rspa.1970.0119. S2CID 122980366.
^Frank, Joachim (2006). Frank, Joachim (ed.). Electron Tomography. doi:10.1007/978-0-387-69008-7. ISBN 978-0-387-31234-7. S2CID 241282825.
^Mastronarde, D. N. (1997). "Dual-Axis Tomography: An Approach with Alignment Methods That Preserve Resolution". Journal of Structural Biology. 120 (3): 343–352. doi:10.1006/jsbi.1997.3919. PMID 9441937.
^Y. Yang; et al. (2017). "Deciphering chemical order/disorder and material properties at the single-atom level". Nature. 542 (7639): 75–79. arXiv:1607.02051. Bibcode:2017Natur.542...75Y. doi:10.1038/nature21042. PMID 28150758. S2CID 4464276.
^Scott, M. C.; Chen, C. C.; Mecklenburg, M.; Zhu, C.; Xu, R.; Ercius, P.; Dahmen, U.; Regan, B. C.; Miao, J. (2012). "Electron tomography at 2.4-ångström resolution" (PDF). Nature. 483 (7390): 444–7. Bibcode:2012Natur.483..444S. doi:10.1038/nature10934. PMID 22437612. S2CID 1600103.
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distances at which they are found. The atomic electrontomography technique is performed in transmission electron microscopes capable of reaching sub-Angstrom...
University host and University of Copenhagen co-host). Cryogenic electrontomography (cryo-ET), a specialized application where many images are taken...
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contribute to chloroplasts' large surface area to volume ratio. A recent electrontomography study of the thylakoid membranes has shown that the stroma lamellae...
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nucleus is converted into a neutron while releasing a positron and an electron neutrino (νe). Positron emission is mediated by the weak force. The positron...
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Positron emission tomography (PET) is a functional imaging technique that uses radioactive substances known as radiotracers to visualize and measure changes...
cellular structures characteristic of eukaryotes. In 2022, cryo-electrontomography demonstrated that Asgard archaea have a complex actin-based cytoskeleton...
model known as the 'bifurcation model', which was based on the first electrontomography study of plant thylakoid membranes, depicts the stromal membranes...
microscopy. In 2012, Miao applied the CDI method to pioneer atomic electrontomography (AET), enabling the first determination of 3D atomic structures without...
interests are in electrontomography, electron holography, energy-filtered imaging and ab initio structure determination by electron diffraction. During...
this number continues to grow. An application of cryo-EM is cryo-electrontomography (cryo-ET), where a 3D reconstruction of the sample is created from...
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