Global InformationDCL2 information
| Endoribonuclease Dicer homolog 2 | |||||||
|---|---|---|---|---|---|---|---|
 Cartoon representation of Arabidopsis DCL2, Based on computational predictions using Alphafold2 and rendered with open software Mol Star * (https://alphafold.ebi.ac.uk/entry/Q3EBC8, https://molstar.org/viewer/) | |||||||
| Identifiers | |||||||
| Organism | |||||||
| Symbol | DCL2 | ||||||
| Alt. symbols | AT3G03300 | ||||||
| UniProt | Q3EBC8 | ||||||
| |||||||
DCL2 (an abbreviation of Dicer-like 2) is a gene in plants that codes for the DCL2 protein, a ribonuclease III enzyme involved in processing exogenous double-stranded RNA (dsRNA) into 22 nucleotide small interference RNAs (siRNAs).[1]
Diverse sources of dsRNAs have been characterized, broadly classified as exogenous or endogenous. A classical example of exogenous derived dsRNAs are the viral genomes release during infection, specially from those double-stranded RNA viruses, where the cleavage of dsRNA produce small RNA products called viral siRNAs or vsi-RNAs.[2] Other examples of exogenous source of dsRNAs are transgenic with several insertion loci along the plant hos genome.[3] DCL2 also process endogenous sources as double-stranded RNAs derived of cis-natural antisense transcripts, generating 22nt short interfering RNA (natsi-RNAs); however, the biological relevance, evolutionary conservation, and experimental validation of natsi-RNAs remains controversial.[4]
- ^ Jin L, Chen M, Xiang M, Guo Z (February 2022). "RNAi-Based Antiviral Innate Immunity in Plants". Viruses. 14 (2): 432. doi:10.3390/v14020432. PMC 8875485. PMID 35216025.
- ^ Sanan-Mishra N, Abdul Kader Jailani A, Mandal B, Mukherjee SK (2021-03-02). "Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology". Frontiers in Plant Science. 12: 610283. doi:10.3389/fpls.2021.610283. PMC 7960677. PMID 33737942.
- ^ Stam M (January 1997). "Review Article: The Silence of Genes in Transgenic Plants". Annals of Botany. 79 (1): 3–12. doi:10.1006/anbo.1996.0295.
- ^ Meyers BC, Zhan J, Shevela D, Slotkin KR (2022), Plant Small RNAs: Biogenesis and Functions, Agrisera Educational Poster Collection, Poster 6, 2022, Figshare, doi:10.6084/m9.figshare.21186073, retrieved 2022-11-06