The colloidal gold protein assay is a highly sensitive biochemical assay for determining the total concentration of protein in a solution (~0.1 ng/μL to 200 ng/μL).[1] It was first described in 1987 by two groups who used commercially available "Aurodye" colloidal gold solutions.[2][3] Notably, the formulation of Aurodye changed between 1987 and 1990 such that it became incompatible with protein assays,[4] however vendors such as Bio-Rad[5] & Diversified Biotech[4] starting offering colloidal gold formulations that were suitable for protein assays. These products have since been discontinued and there are no vendors that currently explicitly sell colloidal gold for the assay, however detailed synthetic procedures were published to produce the ~17-40 nm gold nanoparticles that are suitable for the assay, along with modifications to increase the shelf stability of the colloidal gold & adapt the assay to microplate format & increase its sensitivity.[6] Gold nanoparticles in the ~17-40 nm size range that are presumably compatible with the assay are currently commercially available.[7]
^Ahmed, Hafiz (2005). Principles and Reactions of Protein Extraction, Purification, and Characterization. CRC Press. p. 58. doi:10.1201/9780203507438. ISBN 978-0-203-50743-8.
^Stoscheck, Christa M. (1987). "Protein assay sensitive at nanogram levels". Analytical Biochemistry. 160 (2). Elsevier BV: 301–305. doi:10.1016/0003-2697(87)90051-0. ISSN 0003-2697.
^Hunter, John B.; Hunter, Susan M. (1987). "Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye". Analytical Biochemistry. 164 (2). Elsevier BV: 430–433. doi:10.1016/0003-2697(87)90515-x. ISSN 0003-2697.
^ abDeutscher, M.P. (1990). Guide to Protein Purification. Methods in enzymology. Academic Press. p. 64. ISBN 978-0-12-182083-1. Retrieved 2023-04-09.
^Rad, Bio-. "Enhanced Colloidal Gold Total Protein Detection Kit - Instruction Manual" (PDF). Bulletin_9238.pdf. Bio-Rad. Retrieved 9 April 2023.
^Ciesiolka, Thomas; Gabius, Hans-Joachim (1988). "An 8- to 10-fold enhancement in sensitivity for quantitation of proteins by modified application of colloidal gold". Analytical Biochemistry. 168 (2). Elsevier BV: 280–283. doi:10.1016/0003-2697(88)90319-3. ISSN 0003-2697.
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The Bradford proteinassay (also known as the Coomassie proteinassay) was developed by Marion M. Bradford in 1976. It is a quick and accurate spectroscopic...
ratio <0.8) Bradford proteinassay: Detection in the range of ~1 mg/mL Biuret Test Derived Assays: Bicinchoninic acid assay (BCA assay): Detection down to...
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fabricate quantum dots. Possible methods include colloidal synthesis, self-assembly, and electrical gating. Colloidal semiconductor nanocrystals are synthesized...
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raised antiserum to these specific HNL, which were then applied (with colloidalgold particles in tow) to Black Cherry cotyledon and endosperm. Here it was...
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visco-elastic properties of biopolymers, and study cell motility. A bio-molecular assay in which clusters of ligand coated nano-particles are both optically trapped...
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morphological parameters, viability and both extracellular and intracellular protein expression. The homogeneous cell population obtained after sorting can...
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Martina; Fukal, Ladislav; Rauch, Pavel (2010-07-01). "Development of colloidal carbon-based immunochromatographic strip for rapid detection of carbaryl...
Studies in Interface Science, Introduction to Molecular-Microsimulation of Colloidal Dispersions, vol. 17, Elsevier, pp. 19–63, doi:10.1016/S1383-7303(03)80031-5...
There's Plenty of Room at the Bottom. British Society for Nanomedicine Colloidalgold Heart nanotechnology IEEE P1906.1 – Recommended Practice for Nanoscale...
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