A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on proliferating tumor cells[1] as well as for titration of Cell-killing Particles (CKPs) in virus stocks.[2] It was first developed by T.T. Puck and Philip I. Marcus at the University of Colorado in 1955.[3]
Although this technique can provide accurate results, the assay is time-consuming to set up and analyze and can only provide data on tumor cells that can grow in culture. The word "clonogenic" refers to the fact that these cells are clones of one another.
^Hoffman, Robert M. (1991). "In vitro sensitivity assays in cancer: A review, analysis, and prognosis". Journal of Clinical Laboratory Analysis. 5 (2): 133–43. doi:10.1002/jcla.1860050211. PMID 2023059.
^Ngunjiri, J. M.; Sekellick, M. J.; Marcus, P. I. (2008). "Clonogenic Assay of Type a Influenza Viruses Reveals Noninfectious Cell-Killing (Apoptosis-Inducing) Particles". Journal of Virology. 82 (6): 2673–80. doi:10.1128/JVI.02221-07. PMC 2258965. PMID 18184709.
^Transcript of TWiV interview@http://www.twiv.tv/TWiV197-082612.pdf
A clonogenicassay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently...
viability assays: resazurin method, ATP test, Ethidium homodimer assay (detect dead or dying cells), Bacteriological water analysis, Clonogenicassays, ......
basic effects of a control treatment (such as a dose-survival curve in clonogenicassay) Color Curve fitting Linear regression Logarithmic scale Protein Serial...
(SRB) assay, WST assay and clonogenicassay. Suitable assays can be combined and performed sequentially on the same cells in order to reduce assay-specific...
depicts its main separation from "frogging". Calcein AM Clonogenicassay Ethidium homodimer assay Evans blue Fluorescein diacetate hydrolysis/Propidium...
of stem cells can be illustrated in vitro, using methods such as clonogenicassays, in which single cells are assessed for their ability to differentiate...
of clonogenic survival Long-lasting or irreversible cell cycle arrest that leads to cell death can't be distinguished by this method. Clonogenicassays...
be illustrated with relative ease in vitro, using methods such as clonogenicassays, where the progeny of a single cell is characterized. However, it...
vivo clonogenicassay for hepatocytes, and used it to quantify their survival when exposed to X-rays and neutrons. Jirtle also used this clonal assay to...
cartilage or myogenic (muscle) tissue and bone. An ex vivo assay for examining the clonogenic potential of multipotent marrow cells was later reported in...
with the understanding of biological features of MSCs. Differentiation assays are used to identify MSCs, and the mesenchymal potential of OP9 cells was...
increases in mutagenesis and a reduction in the survival of cells in clonogenicassays. X-rays and gamma rays were reported to cause increases in DNA double...
tumor cancer stem-like cell followed in 2002 with the discovery of a clonogenic, sphere-forming cell isolated and characterized from adult human brain...
anticancer drugs may directly improve their survival and sustain their clonogenicity during exposure to cytostatic drugs, allowing disease recurrence when...
impaired granulomonocytopoiesis due to FANCA mutation. Studies using clonogenic myeloid progenitors (CFU-GM) have also shown that the frequency of CFU-GM...
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