Bicinchoninic acid information

Bicinchoninic acid
Names
	Preferred IUPAC name [2,2′-Biquinoline]-4,4′-dicarboxylic acid
	Other names Bicinchoninic acid; 4,4′-Dicarboxy-2,2′-biquinoline; 2,2'-Biquinoline-4,4'-dicarboxylic acid
Identifiers
CAS Number	1245-13-2 (free acid) ; 979-88-4 (disodium salt) ;
3D model (JSmol)	Interactive image;
ChEMBL	ChEMBL431482 ;
ChemSpider	64223 ;
ECHA InfoCard	100.013.628
PubChem CID	71068;
UNII	CX56TX9Y1I ;
CompTox Dashboard (EPA)	DTXSID8061638 ;
	InChI InChI=1S/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26) Key: AFYNADDZULBEJA-UHFFFAOYSA-N ;
	SMILES O=C(O)c1cc(nc2c1cccc2)c3nc4ccccc4c(c3)C(=O)O;
Properties
Chemical formula	C20H12N2O4
Molar mass	344.326 g·mol−1
Appearance	Cream colored powder
Odor	Characteristic odor[vague]
Melting point	352 °C (666 °F; 625 K) decomposes
Solubility in water	Partially soluble in cold water, hot water[vague]
Hazards
	Occupational safety and health (OHS/OSH):
Main hazards	Will irritate eyes and mucous membranes.
NFPA 704 (fire diamond)	1
Safety data sheet (SDS)	External MSDS
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Bicinchoninic acid /baɪsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic is an organic compound with the formula (C₉H₅NCO₂H)₂. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.^[1]^[2]^[3]

Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu⁺ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.^[4]

^ Smith, P.K.; Krohn, R.I.; Hermanson, G.T.; Mallia, A.K.; Gartner, F.H.; Provenzano, M.D.; Fujimoto, E.K.; Goeke, N.M.; Olson, B.J.; Klenk, D.C. (1985). "Measurement of Protein Using Bicinchoninic Acid". Analytical Biochemistry. 150 (1): 76–85. doi:10.1016/0003-2697(85)90442-7. PMID 3843705.
^ Brown, Rhoderick E.; Jarvis, Kari L.; Hyland, Kristi J. (1989). "Protein measurement using bicinchoninic acid: Elimination of interfering substances". Analytical Biochemistry. 180 (1): 136–139. doi:10.1016/0003-2697(89)90101-2. PMID 2817336.
^ Noble, James E.; Bailey, Marc J.A. (2009). "Chapter 8 Quantitation of Protein". Guide to Protein Purification, 2nd Edition. Methods in Enzymology. Vol. 463. pp. 73–95. doi:10.1016/S0076-6879(09)63008-1. ISBN 9780123745361. PMID 19892168.
^ Pierce BCA Protein Assay Kit

[1] Smith, P.K.; Krohn, R.I.; Hermanson, G.T.; Mallia, A.K.; Gartner, F.H.; Provenzano, M.D.; Fujimoto, E.K.; Goeke, N.M.; Olson, B.J.; Klenk, D.C. (1985). "Measurement of Protein Using Bicinchoninic Acid". Analytical Biochemistry. 150 (1): 76–85. doi:10.1016/0003-2697(85)90442-7. PMID 3843705.

[2] Brown, Rhoderick E.; Jarvis, Kari L.; Hyland, Kristi J. (1989). "Protein measurement using bicinchoninic acid: Elimination of interfering substances". Analytical Biochemistry. 180 (1): 136–139. doi:10.1016/0003-2697(89)90101-2. PMID 2817336.

[3] Noble, James E.; Bailey, Marc J.A. (2009). "Chapter 8 Quantitation of Protein". Guide to Protein Purification, 2nd Edition. Methods in Enzymology. Vol. 463. pp. 73–95. doi:10.1016/S0076-6879(09)63008-1. ISBN 9780123745361. PMID 19892168.

[4] Pierce BCA Protein Assay Kit

Bicinchoninic acid information

and 15 Related for: Bicinchoninic acid information

Bicinchoninic acid assay

Bicinchoninic acid

Virus quantification

Biuret test

BCA

Dumas method

Protein purification

Assay

Kjeldahl method

Lowry protein assay

Chemical test

Pierce Biotechnology

Protein methods

Protein adsorption

Pierce Protein Assay

Names

Preferred IUPAC name [2,2′-Biquinoline]-4,4′-dicarboxylic acid
Other names Bicinchoninic acid 4,4′-Dicarboxy-2,2′-biquinoline 2,2'-Biquinoline-4,4'-dicarboxylic acid
Identifiers
CAS Number	1245-13-2 (free acid)^Y 979-88-4 (disodium salt)^N
3D model (JSmol)	Interactive image
ChEMBL	ChEMBL431482^Y
ChemSpider	64223^Y
ECHA InfoCard	100.013.628
PubChem CID	71068
UNII	CX56TX9Y1I^Y
CompTox Dashboard (EPA)	DTXSID8061638
InChI InChI=1S/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26)^Y Key: AFYNADDZULBEJA-UHFFFAOYSA-N^Y
SMILES O=C(O)c1cc(nc2c1cccc2)c3nc4ccccc4c(c3)C(=O)O
Properties
Chemical formula	C₂₀H₁₂N₂O₄
Molar mass	344.326 g·mol⁻¹
Appearance	Cream colored powder
Odor	Characteristic odor^[vague]
Melting point	352 °C (666 °F; 625 K) decomposes
Solubility in water	Partially soluble in cold water, hot water^[vague]
Hazards
Occupational safety and health (OHS/OSH):
Main hazards	Will irritate eyes and mucous membranes.
NFPA 704 (fire diamond)	1
Safety data sheet (SDS)	External MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Y verify (what is ^YN ?) Infobox references