The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques. It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature, cited over 300,000 times.[1][2][3]
^Van Noorden, R.; Maher, B.; Nuzzo, R. (2014). "The top 100 papers". Nature. 514 (7524): 550–553. Bibcode:2014Natur.514..550V. doi:10.1038/514550a. PMID 25355343.
^Kresge, N.; Simoni, R. D.; Hill, R. L. (2005). "The Most Highly Cited Paper in Publishing History: Protein Determination by Oliver H. Lowry". Journal of Biological Chemistry. 280 (28): e25.
^Garfield, E. (1990). "The Most-Cited Papers of All Time, SCI 1945-1988. Part 1A. The SCI Top 100—Will the Lowry Method Ever Be Obliterated?" (PDF). Current Contents. 7: 3–14.
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The Lowryproteinassay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by...
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proteinassay: Detection in the range of ~1 mg/mL Biuret Test Derived Assays: Bicinchoninic acid assay (BCA assay): Detection down to 0.5 μg/mL Lowry...
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the Folin-Ciocalteu reagent, folins phenol reagent used in the Lowryproteinassay and EPTA, ethanolic phosphotungstic acid. Phosphomolybdic acid Phosphotungstic...
Tinbergen publishes The Study of Instinct. May 28 – Oliver H. Lowry submits his Lowryproteinassay procedure for publication. October 15 – The progestin norethisterone...
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accurate protein quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not...
Derr (March 1, 1978). "A linear Lowry-Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins". Analytical Biochemistry. 85 (1):...
concentration of a protein can be determined by measuring the OD at 280 nm on a spectrophotometer, which can be used with a standard curve assay to quantify...
methods widely used for measuring the amount of protein adsorbed on surfaces include radio-labelling, Lowryassay, scanning angle reflectometry, total internal...
choose to activate dyes in acid dye-baths instead. According to the Brønsted–Lowry acid–base theory, an acid is a molecule or ion capable of donating a proton...
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most-cited paper in history is a paper by Oliver Lowry describing an assay to measure the concentration of proteins. By 2014 it had accumulated more than 305...
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