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Autoradiograph information


Autoradiography of a coronal brain slice, taken from an embryonal rat. GAD67-binding marker is highly expressed in the subventricular zone.

An autoradiograph is an image on an X-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors[1] or rare-earth phosphorimaging systems.[2] The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is marked using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being metabolized by the hornwort plant or by epiphytic microorganisms in the biofilm layer surrounding the plant.[3]

  1. ^ Barthe N, Coulon P, Hennion C, Ducassou D, Basse-Cathalinat B, Charpak G (May 1999). "Optimization of a new scintillation gas detector used to localize electrons emitted by 99mTc". J Nucl Med. 40 (5): 868–75. PMID 10319763.
  2. ^ Encyclopedia of Life Sciences: Phosphorimager
  3. ^ Rupassara, S. I., R.A. Larson, G.K. Sims, and K.A. Marley. 2002 Degradation of atrazine by hornwort in aquatic systems. Bioremediation Journal 6(3): 217-224.

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Autoradiograph

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An autoradiograph is an image on an X-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from...

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Radioactivity in the life sciences

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Autoradiograph of a coronal brain tissue slice, with a radiolabeled GAD67 probe. Most intense signal is seen in subventricular zone....

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Gene expression

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to the target sequence. The radiolabeled RNA is then detected by an autoradiograph. Because the use of radioactive reagents makes the procedure time-consuming...

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Biomedicine

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radioactivity of the probes creates black bands on the film, called an autoradiograph. As a result, only similar patterns of DNA to that of the probe are...

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Scintigraphy

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Plantes. He presented the results in 1950 under the name 'indirect Autoradiograph’. In 1970, the Physikalisch-Medizinische Gesellschaft für Neuroradiologie...

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Primer extension

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transcript by the intensity of the transcript band on the resulting autoradiograph. Unlike S1 mapping, however, primer extension can only be used to locate...

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Spent nuclear fuel

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Imaging Autoradiograph RadBall Scintigraphy Single-photon emission (SPECT) Positron-emission tomography (PET) Therapy Fast-neutron Neutron capture therapy...

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Circular chromosome

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the theta replication model of circular bacterial chromosomes. See Autoradiograph of intact replicating chromosome of E.coli [1] As described above, bacterial...

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Nuclear microreactor

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Imaging Autoradiograph RadBall Scintigraphy Single-photon emission (SPECT) Positron-emission tomography (PET) Therapy Fast-neutron Neutron capture therapy...

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Radiography

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rentogen (レントゲン), shares its etymology with the original English term. Autoradiograph Background radiation Computer-aided diagnosis GXMO Imaging science List...

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Operation Crossroads

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be anywhere. The following day, August 10, Warren showed Blandy an autoradiograph of a fish, an x-ray picture made by radiation coming from the fish....

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Index of genetics articles

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Assembly Assortative mating Atavism ATP Attenuator Autogamy Autopolyploid Autoradiograph Autoradiography Autosomal dominant Autosome Autotroph Auxotroph Auxotrophic...

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Restriction landmark genomic scanning

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yielding a visual representation of the results in the form of an autoradiograph. The same combination of restriction enzymes will produce the same pattern...

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Theta structure

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cells with a pulse of tritiated thymidine, quenching rapidly and then autoradiographing. Results showed that the radioactive thymidine was incorporated into...

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Isotopic labeling

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radioactive decay can be detected through an ionization chamber or autoradiographs of gels. An example of the use of isotopic labeling is the study of...

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Barbara Askins

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second emulsion to this radiation." The resulting print, known as an autoradiograph, reproduces the image with significant increases in density and contrast...

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Alma Howard

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Research Unit, she met physicist Stephen Pelc who had developed a variant autoradiograph technique and "was much interested in his use of radioactive iodine...

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Patent drawing

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invention. For example, photographs of electrophoresis gels, blots, autoradiographs, cell cultures, histological tissue cross sections, animals, plants...

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Proliferative index

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update more radiolabeled Thymidine, which produces darker spots on the autoradiograph film. A bromodeoxyuridine assay, similar to thymidine-labeling indexing...

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Identification of cell death

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electron microscope was employed by Hodges and Muir (1975) to study autoradiograph. This approach was combined with the cytochemical method for demonstrating...

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Gemistocyte

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anaplasia and degeneration. Tumor and brain tissue can be examined using autoradiographs, showing if the gemistocytic astrocytes could be labeled by the tritiated...

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Agfacontour Professional

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Phototechnik und Wirtschaft (1971) Nr.2, p 39 Myšák, F "The Evaluating of Autoradiographs by Means of the Film Agfacontour Professional" Yurow, Harvey W. "A...

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Ekkehard Kallee

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articles about the detection method with 131I-marked insulin. By taking autoradiographs of capillary electrophoresis strips he could detect down to 10−9 gram...

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Obsolete models of DNA structure

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initially questions about how it might affect DNA replication. In 1963, autoradiographs of the E. coli chromosome demonstrated that it was a single circular...

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National Security and Nuclear Diplomacy

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Imaging Autoradiograph RadBall Scintigraphy Single-photon emission (SPECT) Positron-emission tomography (PET) Therapy Fast-neutron Neutron capture therapy...

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Equidensitometry

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1. Auflage 1974 (in German) Myšák, F. (1973). "The Evaluating of Autoradiographs by Means of the Film Agfacontour Professional". Journal Isotopenpraxis...

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