Global InformationRubicon homology domain information
| Rubicon homology domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 RH domain of human Rubicon (red) bound to Rab7-GTP (grey) (PDB: 6WCW) | |||||||||
| Identifiers | |||||||||
| Symbol | RH Domain | ||||||||
| Pfam | PF13901 | ||||||||
| Pfam clan | CL0229 | ||||||||
| InterPro | IPR025258 | ||||||||
| SMART | SM01175 | ||||||||
| SCOP2 | 6WCW / SCOPe / SUPFAM | ||||||||
| |||||||||
The Rubicon homology domain (also known as RH domain) is an evolutionarily conserved protein domain of approximately 250 amino acids that mediates protein–protein interaction.[1] RH domains are present in several human proteins involved in regulation of autophagy and endosomal trafficking.[2] While not all RH domains have been characterized, those of human Rubicon and PLEKHM1 mediate interaction with the small GTPase Rab7, which is found on late endosomes and autophagosomes.[2][3]
RH domains contain 16 conserved cysteine and histidine residues that bind zinc atoms and form at least 4 zinc finger motifs.[2][3] Amino acid residues toward the C-terminus of the RH domain of Rubicon have been shown to be essential for interaction with Rab7.[2]
- ^ "Pfam: Family: zf-RING_9 (PF13901)". pfam.xfam.org. Retrieved 2022-05-31.
- ^ a b c d Bhargava HK, Tabata K, Byck JM, Hamasaki M, Farrell DP, Anishchenko I, et al. (July 2020). "Structural basis for autophagy inhibition by the human Rubicon-Rab7 complex". Proceedings of the National Academy of Sciences of the United States of America. 117 (29): 17003–17010. doi:10.1073/pnas.2008030117. PMC 7382272. PMID 32632011.
- ^ a b Tabata K, Matsunaga K, Sakane A, Sasaki T, Noda T, Yoshimori T (December 2010). "Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain". Molecular Biology of the Cell. 21 (23): 4162–4172. doi:10.1091/mbc.E10-06-0495. PMC 2993745. PMID 20943950.