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Streak plates of several bacterial species on nutrient agar plates
Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume):[1]
0.5% peptone - this provides organic nitrogen
0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts
1.5% agar - this gives the mixture solidity
0.5% sodium chloride - this gives the mixture proportions similar to those found in the cytoplasm of most organisms
distilled water - water serves as a transport medium for the agar's various substances
pH adjusted to neutral (6.8) at 25 °C (77 °F).
Nutrient broth has the same composition, but lacks agar.[2][3]
These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes. Then they are cooled to around 50 °C (122 °F) and poured into Petri dishes which are covered immediately. Once the dishes hold solidified agar, they are stored upside down and are often refrigerated until used. Inoculation takes place on warm dishes rather than cool ones: if refrigerated for storage, the dishes must be rewarmed to room temperature prior to inoculation.[citation needed]
^American Public Health Association, American Chemical Society, Association of Official Agricultural Chemists (1920). Standard Methods for the Examination of Water and Sewage. American public health association. pp. 95.{{cite book}}: CS1 maint: multiple names: authors list (link)
Nutrientagar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 0...
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automatic pipette, delivering drops of 20μl. Petri dishes containing nutrientagar or other appropriate medium. Phosphate Buffered Saline (PBS) or other...
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axenic media or on non-nutrientagar plates coated with bacteria. Escherichia coli can be used to overlay the non-nutrientagar plate and a drop of cerebrospinal...
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potato dextrose agar or potato dextrose broth, Wallerstein Laboratories nutrientagar, yeast peptone dextrose agar, and yeast mould agar or broth. Home...
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the pathogen should then be cultured on reduced nutrientagar. Inhibiting contaminant growth on the agar by adding antibiotics and keeping the temperature...
best on heart infusion agar supplemented with 5% horse blood. It is also able to grow on TSA, NutrientAgar, Mac Conkey or CASA Agar. Pseudomonas luteola...
counter stained red by Safranin. The optimal growth temperature on a NutrientAgar plate is 30-35 degree Celsius. The Genus Burkholderia (including B....
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