This article is an orphan, as no other articles link to it. Please introduce links to this page from related articles; try the Find link tool for suggestions. (January 2017)
NUN buffer is a solution that makes it possible to purify proteins located in the nucleus of eukaryotic cells.[1] Although other procedures are available[2] they result in loss of albumin D-box binding protein (DBP) which is unwanted if nuclear signal pathways are to be investigated. Therefore, a new extraction procedure was developed in 1993 to increase recovery of nonhistone proteins using a (NUN) solution containing 0.3 M NaCl, 1 M urea, and 1% nonionic detergent Nonidet P-40, which destabilize salt bridges, hydrogen bonds, and hydrophobic interactions, respectively; resulting in a disruption of interaction between proteins and DNA. By incubating nuclei in NUN buffer and centrifuging the solution, the supernatant will therefore contain nuclear proteins.
^Lavery, Daniel J.; Schibler, Ueli (1993). "Circadian transcription of the cholesterol 7 alpha hydroxylase gene may involve the liver-enriched bZIP protein DBP". Genes & Development. 7 (10): 1871–84. doi:10.1101/gad.7.10.1871. PMID 8405996.
^Gorski, K.; Carneiro, M.; Schibler, U. (1986). "Tissue-specific in vitro transcription from the mouse albumin promoter". Cell. 47 (5): 767–76. doi:10.1016/0092-8674(86)90519-2. PMID 3779841. S2CID 26448485.
NUNbuffer is a solution that makes it possible to purify proteins located in the nucleus of eukaryotic cells. Although other procedures are available...
"Princess of Panty Shields", "Empress of Elvis Impersonators," and the "Buffer of Foreheads." Her routine, which blended observational and insult humor...
%96 "F:4< qC@H? u@I yF>AD ~G6C %96 {2KJ s@8 In Emacs, one can ROT13 the buffer or a selection with the commands M-x toggle-rot13-mode, M-x rot13-other-window...
oil extraction by 2045. In October 2021, he proposed a 3,200-foot (980 m) buffer between new fossil fuel extraction sites and densely populated areas. In...
Championship. Within the household, Peggy provides a voice of reason and a buffer between Hank and Bobby, who have difficulty relating to each other. Peggy...
interconnections,[citation needed] but with buffer and hook couplings on the outer ends of the set of coaches suitable for buffer and chain screw coupling Two complete...
Simon handed his county of the Vexin over to King Philip. The Vexin was a buffer state between Normandy and the lands of the French king, and Simon had been...
River. The southern edge is set back about 10 km from the Gulf of Guinea, buffered by the Central African mangroves ecoregion where the effects of salt water...
derived from the Seneca name spelled variously Kanandarque, Ganondagan, Ga-nun-da-gwa, or in a modern transcription, tganǫdæ:gwęh, which means "the chosen...
Cuoco, Christopher Murray, Mari Morrow, Heidi Schanz, Traci Lords, Michael Buffer, Michael B. Silver, Danny Goldring, Rolando Molina, Gary Anthony Sturgis...
and B12), glucuronolactone and simple sugars (sucrose and glucose) in a buffer solution of carbonated water, sodium bicarbonate and magnesium carbonate...
but each attempt ended in failure. The Ottomans regarded Wallachia as a buffer zone between them and the Kingdom of Hungary and for a yearly tribute did...
year. By immunizing wild or stray animals, ORV programs work to create a buffer zone between the rabies virus and potential contact with humans, pets, or...
(SGNP). It is classified as mixed moist deciduous type forest. It acts as a buffer between SGNP and the city, being one of the few green spaces (spread over...
when he was six, Gama was put under the care of his maternal grandfather Nun Pahalwan.[citation needed] Following his death, Gama was taken care of by...
Mateo Cruz, on August 28, 2021, as announced on Miocic's Instagram by Bruce Buffer. Miocic can understand Croatian, though he is not fluent and has been learning...
territory is enclosed within walls and surrounded by a park, which forms the buffer zone. The park is limited by the urban fabric of the city on the north and...
managers have made an effort to try to include Sherpas' voices by creating buffer-zone user groups. These groups are made up of political leaders from the...
rates and achieving the real-time computing performance required to prevent buffer underruns and enable smooth streaming of the content. However, computer...
Inanna of the evening (Sumerian: ᵈinanna-sig), and Inanna (Sumerian: ᵈinanna-NUN). The names of four temples in Uruk at this time are known, but it is impossible...
exclaves within its buffer zone, features a number of landmarks. The main zone is that of the Main Square and Historic Centre (139.35 ha; buffer zone: 284.93...
the ruined Suez Canal; and the Emirate of Afghanistan, which serves as a buffer between the Raj and Russia. In the now long-destroyed United States, several...
Global Information