MetaboAnalyst[1][2][3] is a set of online tools for metabolomic data analysis and interpretation, created by members of the Wishart Research Group at the University of Alberta. It was first released in May 2009 and version 2.0 was released in January 2012. MetaboAnalyst provides a variety of analysis methods that have been tailored for metabolomic data. These methods include metabolomic data processing, normalization, multivariate statistical analysis, and data annotation. The current[vague] version is focused on biomarker discovery and classification.
MetaboAnalyst supports a wide variety of data input types commonly generated by metabolomic studies including GC/LC-MS raw spectra, MS/NMR peak lists, NMR/MS peak intensity table, NMR/MS spectral bins, and metabolite concentrations.
MetaboAnalyst has four modules:
Data processing
Statistical analysis (one-factor, two-factor, and time-series data)
Functional enrichment analysis
Metabolic pathway analysis
The table below summarizes the main features of each functional module.[4]
1. Data Processing
2. Statistical Analysis
3. Functional Enrichment Analysis
Peak detection
Univariate analysis
Over representation analysis
Retention time correction
Dimension reduction
Single sample profiling
Peak alignment
Feature selection
Quantitative enrichment analysis
Baseline filtering
Cluster analysis
Data integrity check
Classification
4. Metabolic Pathway Analysis
Missing value imputation
Two-way ANOVA
Enrichment analysis
ASCA
Topology analysis
Temporal comparison
Interactive visualization
MetaboAnalyst generates a PDF report that includes a written record of each analysis step and displays results in graphical and tabular format. Users can also download processed data files and PNG image files.
MetaboAnalyst is part of a suite of metabolomics databases that also includes Human Metabolome Database (HMDB),[5][6][7] DrugBank,[8][9][10] Toxin and Toxin-Target Database,[11] and The Small Molecule Pathway Database.[12] The HMDB has over 7900 human metabolites and roughly 7200 associated DNA and protein sequences, that are linked to these metabolite entries. While DrugBank includes information on 6707 drugs and 4228 non-redundant drug targets, enzymes, transporters, and carriers, T3DB houses over 2900 common toxins and environmental pollutants. The suite is rounded out by SMPDB with its pathway diagrams for more than 350 human metabolic and disease pathways.
^Xia, J; Psychogios, N; Young, N; Wishart, DS (Jul 2009). "MetaboAnalyst: a web server for metabolomic data analysis and interpretation". Nucleic Acids Research. 37 (Web Server issue): W652-60. doi:10.1093/nar/gkp356. PMC 2703878. PMID 19429898.
^Xia, J; Wishart, DS (Jun 2011). "Metabolomic data processing, analysis, and interpretation using MetaboAnalyst". Current Protocols in Bioinformatics. Chapter 14: Unit 14.10. doi:10.1002/0471250953.bi1410s34. PMID 21633943.
^Xia, J; Wishart, DS (Jun 2011). "Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst". Nature Protocols. 6 (6): 743–60. doi:10.1038/nprot.2011.319. PMID 21637195.
^"MetaboAnalyst: Overview". Archived from the original on 1 December 2011. Retrieved 17 January 2012.
^Forsythe, IJ; Wishart, DS (Mar 2009). "Exploring human metabolites using the human metabolome database". Current Protocols in Bioinformatics. Chapter 14: Unit14.8. doi:10.1002/0471250953.bi1408s25. PMID 19274632.
^Wishart, DS; Knox, C; Guo, AC; Shrivastava, S; Hassanali, M; Stothard, P; Chang, Z; Woolsey, J (2006-01-01). "DrugBank: a comprehensive resource for in silico drug discovery and exploration". Nucleic Acids Research. 34 (Database issue): D668-72. doi:10.1093/nar/gkj067. PMC 1347430. PMID 16381955. DrugBank
^Wishart, DS; Knox, C; Guo, AC; Cheng, D; Shrivastava, S; Tzur, D; Gautam, B; Hassanali, M (Jan 2008). "DrugBank: a knowledgebase for drugs, drug actions and drug targets". Nucleic Acids Research. 36 (Database issue): D901-6. doi:10.1093/nar/gkm958. PMC 2238889. PMID 18048412.
MetaboAnalyst is a set of online tools for metabolomic data analysis and interpretation, created by members of the Wishart Research Group at the University...
web server and as part of a larger metabolomics analysis suite called MetaboAnalyst. The MSEA web server is a freely available web server for performing...
its output. In 2011 MetPA functions were expanded and integrated into MetaboAnalyst. This integration allows users to perform a more complete analysis and...
(enzymes and transporters) are linked to these metabolite entries. Each MetaboCard entry contains 130 data fields with 2/3 of the information being devoted...