Czapek medium, also called Czapek's agar (CZA)[1][2] or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It was named after its inventors, Czech botanist Friedrich Johann Franz Czapek (May 16, 1868 – July 31, 1921) and American chemist Arthur Wayland Dox (September 19, 1882 – 1954). It was developed to grow Aspergillus niger[3] and Penicillium camemberti.[4] It works well for many saprophytic fungi and soil bacteria[5] such as species of Aspergillus, Candida, Penicillium, and Paecilomyces.[6]
Aspergillus bertholletius on Czapek agar
Friedrich Czapek's original recipe is as follows:[3]
1000 g distilled water
30 g cane sugar – energy source and sole source of carbon[5]
1 g dipotassium phosphate – buffering agent[5]
0.5 g magnesium sulfate – source of cations[5]
0.5 g potassium chloride – source of essential ions[5]
0.01 g iron sulfate – source of cations[5]
Aspergillus fumigatus on Czapek agar
Arthur Wayland Dox added 2 g of sodium nitrate in his version, to provide a sole source of nitrogen that is inorganic.[4] This makes the medium a selective growth medium as only organisms that can use inorganic nitrogen can grow.[6] Czapek and Dox did not add agar but many recipes add 15 g to make a solid medium.[5][6]
^"Czapek Agar (CZA) Recipe". theLabRat.com. 2005. Archived from the original on 16 March 2015. Retrieved 7 November 2017. Czapek Agar (CZA) Recipe
^"NRRL Medium No. 8 Czapek's Solution Agar ( CZA )" (PDF). ARS (NRRL) Culture Collection. Peoria, IL: ARS Culture Collection National Center for Agricultural Utilization Research. Archived (PDF) from the original on 27 December 2016. Retrieved 7 November 2017. Czapek's Solution Agar ( CZA )
^ abCzapek, F (1901–1902). "Untersuchungen über die Stickstoffgewinnung und Eiweifsbildung der Pflanzen" [Studies on nitrogen production and protein formation of plants] (PDF). Beiträge zur chemischen Physiologie und Pathologie (in German). 1 (12): 538–560. OCLC 1519369. Archived from the original on 28 March 2018. Retrieved 2 October 2017.
^ abDox, Arthur Wayland (1910). "The intracellular enzyms of penicillium and aspergillus: with special reference to those of Penicillium camemberti" (PDF). Bulletin (United States Bureau of Animal Industry). 120: 37. OCLC 22281943. Archived from the original on 6 October 2022. Retrieved 3 October 2017.
^ abcdefg"CZAPEK MEDIUM" (PDF). Thermo Fisher Scientific. Lenexa, KS: Remel. September 20, 2007. Archived (PDF) from the original on 17 November 2017. Retrieved 3 October 2017.
^ abc"CZAPEK-DOX AGAR". Hardy Diagnostics Instructions for Use. Santa Maria, CA: Hardy Diagnostics. Archived from the original on 8 July 2017. Retrieved 3 October 2017.
Czapekmedium, also called Czapek's agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It...
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process...
Friedrich Czapek (1868–1921), Czech botanist who developed Czapekmedium Leopold Eustachius Czapek (1792–1840), Czech-Austrian pianist and composer Czapek & Cie...
inconsistencies in test conditions such as incubation time, temperature, and growth medium. Other extrolites produced by this fungus include: asperazine, pyranoigrin...
of India circumzenithal arc, or upside-down rainbow Czapek agar, or Czapekmedium, a growth medium for propagating fungi This disambiguation page lists...
to grow or grows very poorly on Czapekmedium at 25–26 °C with length never exceeding 2 to 3 mm. Colonies on Czapek's agar with 20% sucrose can reach...
soils, dung, and nuts from the tropics. Species can be isolated using Czapekmedium incubated for 3–4 days at 28-31C. A few species are known human pathogens...
carneus grow rapidly, reaching a diameter of 4–5 cm in 2 weeks on Czapekmedium. Normal growth also occurs on media containing 1% dextrane, and growth...
0-3.5 mm/day in Czapek'smedium and Leonian's medium and a growth rate of 3.0-4.0 mm/day for the tomato paste and oatmeal-based medium, Weitzman & Silvia-Hunter's...
growth rate on malt extract agar (MEA) and restricted growth on Czapekmedium (CZA) and Czapek yeast extract agar (CYA). The appearance of colonies on MEA...
semi-synthetic tetracycline Arthur Wayland Dox, a biologist who popularized Czapek-Dox medium Dox (poet), born Jean Verdi Salomon Razakandrainy (1913–1978), a significant...
keratinases. Sodium chloride is stimulatory to its growth on Czapek'smedium (a growth medium in which sodium nitrate is the sole source of nitrogen and...
(PDA), but it can grow on many different types of growth media including Czapek-Dox agar, lignocellulose agar, and several others. [citation needed] Aspergillus...
digitatum can also grow on a variety of laboratory media. On Czapek Yeast Extract Agar medium at 25 °C, white colonies grow in a plane, attaining a velvety...
generated. A. viridinutans can be cultivated on different medium sources, including both Czapek yeast extract agar (CYA) plates and Malt Extract Agar Oxoid®...
developed techniques for growing the mould on a surface of liquid Czapek-Dox medium. Most laboratory containers did not provide a large, flat area, so...
Jack Cohen, co-founder of the Tesco retail chain Franciszek Czapek, co-partner in Patek, Czapek & Co. Leszek Czarnecki, businessman Irena Eris, businesswoman...
colour and gets darker as it ages on culture media. On Czapek or malt extract agar (MEA) medium at 25 °C (77 °F), colonies have the conditions to grow...
and are white in colour. Colonies can grow up to 2–3.5 cm in diameter on Czapek agar when grown under controlled conditions for a span of 7 days. Optimal...
laboratory experiments. The mould was cultured on a surface of liquid Czapek-Dox medium. Over the course of a few days it formed a yellow gelatinous skin...
spines (length of 3 µm). The mating system is heterothallic. Growth on Czapek Yeast Extract Agar (CYA) and Malt Extract Agar (MEA) produces colonies at...
a pink color. A deep orange yellowish pigment is produced by growth on Czapek glucose agar. A. italicus belongs to the phylum Actinomycetota. It is most...
mucormycosis following the inhalation of fungal spores. Czapek's agar is a suitable growth medium for the propagation of C. echinulata. Cunninghamella echinulata...
colonies mature, the colour becomes grey. The growth of P. spinulosum on czapek dox agar (CZ), malt extract agar (MEA) and Yeast Extract with Supplements...
colonies of C. pallescens differ in morphology depending on the growth medium used. On Czapek Yeast Extract Agar (CYA), colonies of C. pallescens are 50-65mm...
laboratory culture conditions. These fungi require Czapek's agar (CZA), a nutrient-defined medium. Colonies grow rapidly at 37 °C on CZA and initially...
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