Global InformationCobalamin riboswitch information
| Cobalamin riboswitch | |
|---|---|
 Predicted secondary structure and sequence conservation of Cobalamin | |
| Identifiers | |
| Symbol | Cobalamin |
| Rfam | RF00174 |
| Other data | |
| RNA type | Cis-reg; riboswitch |
| Domain(s) | Bacteria |
| SO | SO:0000035 |
| PDB structures | PDBe 6VMY |
Cobalamin riboswitch is a cis-regulatory element which is widely distributed in 5' untranslated regions of vitamin B12 (Cobalamin) related genes in bacteria.[1]
Cobalamin (vitamin B12, coenzyme B12 ) riboswitches are structured RNA elements that regulate adjacent genes related to cobalamin metabolism in response to cobalamin binding. Riboswitches are RNA-based genetic regulatory elements present in the 5’ untranslated region (5'UTR) of primarily bacterial RNA. These switches bind to a ligand, which is generally a metabolite, with high affinity and specificity. Ligand binding mediates allosteric rearrangement of mRNA structure, and this results in modulation of gene expression or translation of mRNA to yield a protein. The cobalamin riboswitch, along with most other riboswitches, are cis-regulatory. This means they regulate genes involved in the same metabolic pathways as the metabolite they bind, which creates regulation through a negative feedback loop. Riboswitches are grouped into classes by the ligand that they bind because the ligand-binding or aptamer domain is highly conserved across species. Riboswitches, including the cobalamin riboswitch, have garnered a lot of attention recently due to their therapeutic and synthetic potential,[2] as well as their interesting structural properties.[3][4][5] As of 2019, cobalamin riboswitches have been identified in over 5000 species of bacteria.[2]
- ^ Nahvi A, Sudarsan N, Ebert MS, Zou X, Brown KL, Breaker RR (September 2002). "Genetic control by a metabolite binding mRNA". Chemistry & Biology. 9 (9): 1043–1049. doi:10.1016/S1074-5521(02)00224-7. PMID 12323379.
- ^ a b Pavlova N, Penchovsky R (July 2019). "Genome-wide bioinformatics analysis of FMN, SAM-I, glmS, TPP, lysine, purine, cobalamin, and SAH riboswitches for their applications as allosteric antibacterial drug targets in human pathogenic bacteria". Expert Opinion on Therapeutic Targets. 23 (7): 631–643. doi:10.1080/14728222.2019.1618274. PMID 31079546. S2CID 153306189.
- ^ Batey RT (April 2015). "Riboswitches: still a lot of undiscovered country". RNA. 21 (4): 560–563. doi:10.1261/rna.050765.115. PMC 4371280. PMID 25780138.
- ^ Aghdam EM, Hejazi MS, Barzegar A (November 2016). "Riboswitches: From living biosensors to novel targets of antibiotics". Gene. 592 (2): 244–259. doi:10.1016/j.gene.2016.07.035. PMID 27432066.
- ^ Panchal V, Brenk R (January 2021). "Riboswitches as Drug Targets for Antibiotics". Antibiotics. 10 (1): 45. doi:10.3390/antibiotics10010045. PMC 7824784. PMID 33466288.