positive regulation of DNA-templated transcription, elongation
osteoblast differentiation
replication fork processing
viral process
mRNA 3'-end processing
RNA splicing
RNA export from nucleus
transport
Sources:Amigo / QuickGO
Orthologs
Species
Human
Mouse
Entrez
10189
21681
Ensembl
ENSG00000183684
ENSMUSG00000025134
UniProt
Q86V81
O08583
RefSeq (mRNA)
NM_005782
NM_011568
RefSeq (protein)
NP_005773
NP_035698
Location (UCSC)
Chr 17: 81.89 – 81.89 Mb
Chr 11: 120.48 – 120.49 Mb
PubMed search
[3]
[4]
Wikidata
View/Edit Human
View/Edit Mouse
Aly/REF export factor, also known as THO complex subunit 4 is a protein that in humans is encoded by the ALYREF gene.[5][6]
The ALYREF gene encodes Aly/REF export factor (ALY; THO complex subunit 4, Tho4; RNA and export factor binding protein 1, Refbp1), a ubiquitously expressed nuclear protein that functions as a molecular chaperone and export adapter involved in nuclear export of spliced and unspliced mRNA. The TRanscription-EXport (TREX) complex, a key player in mRNA export, includes the THO subcomplex, the RNA helicase UAP56, and the RNA-binding protein ALY. In yeast, TREX is recruited co-transcriptionally; in human cells it is recruited during a late step of splicing. The human TREX complex is recruited to a region near the 5' end of mRNA by interaction of ALY and THO with the nuclear cap-binding complex. As a chaperone, ALY promotes dimerization of transcription factors containing basic leucine zipper (bZIP) domains.,[7] thereby promoting transcriptional activation. ALY has key roles in 3'-end processing of polyadenylated mRNAs and in nuclear export of both polyadenylated and non-polyadenylated mRNAs.[8][9] After mRNA binds to ALY, it is apparently transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). The full-length ALY protein (Refbp1-I, 255 amino acids encoded by six exons[10]) has a conserved RNA recognition motif (RRM; amino acids 105-182) flanked by alanine/arginine/glycine-rich sequences; an N-terminal region (amino acids 16-37) is sufficient for RNA binding and interaction with the NXF1-NXT1 heterodimer.[11]
^ abcGRCh38: Ensembl release 89: ENSG00000183684 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000025134 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Shi M, Zhang H, Wu X, He Z, Wang L, Yin S, Tian B, Li G, Cheng H (Sep 2017). "ALYREF mainly binds to the 5' and the 3' regions of the mRNA in vivo". Nucleic Acids Res. 45 (16): 9640–9653. doi:10.1093/nar/gkx597. PMC 5766156. PMID 28934468.
^Shi M, Zhang H, Wu X, He Z, Wang L, Yin S, Tian B, Li G, Cheng H (Mar 2019). "ALYREF links 3'-end processing to nuclear export of non-polyadenylated mRNAs". EMBO J. 38 (9): e99910. doi:10.15252/embj.201899910. PMC 6484419. PMID 30858280.
complex subunit 4 is a protein that in humans is encoded by the ALYREF gene. The ALYREF gene encodes Aly/REF export factor (ALY; THO complex subunit 4...
to ALYREF. UAP56 then dissociates, allowing the heterodimeric export receptor NXF1-NXT1 to bind as it recognizes the mRNA indirectly through ALYREF. Further...
"5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader". Cell Research. 27 (5): 606–625. doi:10.1038/cr.2017.55...